| Product Number |
OAEB00429 |
| Product Page |
www.avivasysbio.com/rhpn2-antibody-oaeb00429.html |
| Name |
RHPN2 Antibody (OAEB00429) |
| Molecular Weight |
77 |
| NCBI Gene Id |
85415 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
rhophilin Rho GTPase binding protein 2 |
| Alias Symbols |
76 kDa RhoB effector protein;GTP-Rho-binding protein 2;P76RBE;RHOBP;rhophilin-2;rhophilin-like Rho-GTPase binding protein. |
| Peptide Sequence |
C-TNKNRQKSAST |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Peck JW, Oberst M, Bouker KB, Bowden E, Burbelo PD.; The RhoA-binding protein, rhophilin-2, regulates actin cytoskeleton organization.; J Biol Chem. 2002 Nov 15;277(46):43924-32.; Pubmed 12221077 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for RHPN2 Antibody (OAEB00429) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. |
| Immunogen |
RHPN2 |
| Uniprot ID |
Q8IUC4 |
| Protein Name |
Rhophilin-2 |
| Protein Accession # |
NP_149094.3 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
RHPN2 |
| Predicted Species Reactivity |
Bovine|Human|Mouse|Rat |
| Application |
IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Horse: 100%; Human: 100%; Mouse: 90%; Rabbit: 100%; Rat: 90% |
| Image 1 | Human Kidney
 | OAEB00429 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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