| Product Number |
OAEB00128 |
| Product Page |
www.avivasysbio.com/cybrd1-antibody-oaeb00128.html |
| Name |
CYBRD1 Antibody (OAEB00128) |
| Molecular Weight |
31.6 |
| NCBI Gene Id |
79901 |
| Host |
Goat |
| Clonality |
Polyclonal |
| Purity |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
cytochrome b reductase 1 |
| Alias Symbols |
CYB561A2;cytochrome b561 family, member A2;DCYTB;duodenal cytochrome b;ferric-chelate reductase 3;FRRS3;plasma membrane ascorbate-dependent reductase CYBRD1. |
| Peptide Sequence |
CRNLALDEAGQRSTM |
| Product Format |
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Reference |
* Atanasova BD, Li AC, Bjarnason I, Tzatchev KN, Simpson RJ.; Duodenal ascorbate and ferric reductase in human iron deficiency.; Am J Clin Nutr. 2005 Jan;81(1):130-3.; Pubmed 15640471 |
| Reconstitution and Storage |
Aliquot and store at -20C. Minimize freezing and thawing. |
| Datasheets/Manuals |
Printable datasheet for CYBRD1 Antibody (OAEB00128) |
| Application Info |
Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot: Experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 31.6kDa according to NP_079119. The detected bands were all successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). |
| Immunogen |
CYBRD1 |
| Uniprot ID |
Q53TN4 |
| Protein Name |
Cytochrome b reductase 1 |
| Protein Accession # |
NP_079119.3 |
| Purification |
Ammonium Sulfate Precipitated; Affinity Purified |
| Gene Symbol |
CYBRD1 |
| Predicted Species Reactivity |
Bovine|Dog|Human|Porcine |
| Application |
IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 85%; Dog: 78%; Goat: 85%; Guinea Pig: 78%; Horse: 85%; Human: 100%; Pig: 92% |
| Image 1 | Human Testis
 | OAEB00128 (2.5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. |
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