| Product Number |
OAEA00009 |
| Product Page |
www.avivasysbio.com/ha-tag-antibody-oaea00009.html |
| Name |
HA Tag Antibody (OAEA00009) |
| Clone |
HA.C5 |
| Isotype |
IgG3 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Purity |
Protein A affinity chromatography from mouse ascites fluid |
| Description |
|
| Alias Symbols |
HA Tag, Anti-HA |
| Product Format |
50 ug at 1mg/mL in 10mM PBS, pH 7.2 |
| Reconstitution and Storage |
Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store at 4C (add 0.05% NaN3) for several days to weeks, and at -20C for longer periods. |
| Datasheets/Manuals |
Printable datasheet for OAEA00009 |
| Specificity |
Recognizes the N-terminal, C-terminal or internal HA-tagged fusion proteins |
| Additional Information |
React: Protein A affinity chromatography from mouse ascites fluid |
| Additional Information |
Sensitivity: WB (with ECL): 1-5ng of HA-tagged fusion proteins |
| Protocol Information |
WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Immunogen |
YPYDVPDYA (HA) synthetic peptide conjugated to KLH |
| Uniprot ID |
P03438 |
| Protein Name |
Hemagglutinin |
| Publications |
Characterization of JAK1 Pseudokinase Domain in Cytokine Signaling. Cancers (Basel). 12, (2019). 31892268
Hyperactivation of Oncogenic JAK3 Mutants Depend on ATP Binding to the Pseudokinase Domain. Front Oncol. 8, 560 (2018). 30560087
Janus kinase 2 activation mechanisms revealed by analysis of suppressing mutations. J Allergy Clin Immunol. 143, 1549-1559.e6 (2019). 30092288 |
| Application |
WB, DB, IP, IS |
| Image 1 | Hek293
 | Sample type:
HEK293
Titer:
1:1000
(1ug/mL).Ab dilution probed against HEK293 cells transfected with HA-tagged protein vector; untransfected (1) and transfected (2). |
|
|
