GST-tag Antibody (OAEA00008)

Data Sheet
 
Product Number OAEA00008
Product Page www.avivasysbio.com/gst-tag-antibody-oaea00008.html
Name GST-tag Antibody (OAEA00008)
Clone GST.B6
Isotype IgG2a
Host Mouse
Clonality Monoclonal
Concentration 1 mg/ml
Product Format Liquid 10mM PBS (pH 7.2) with 0.05% sodium azide
Reconstitution and Storage Store at -20C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Store aliquots at 4C for several days to weeks.
Datasheets/Manuals Printable datasheet for OAEA00008
Specificity Recognizes native and denatured forms of purified GST or GST fusion proteins
Application Info WB (with ECL): 1:1000~3000 (incubate at 1 hour in room temperature)
IP: 1:50~200
IS: 1:500~2000. For best results with other assays (Dot, ELISA, etc), please determine optimal working dilution by titration test.
Immunogen GST protein
Sensitivity WB (with ECL): 10-50 ng of purified GST or GST fusion proteins
Purification Protein A affinity chromatography from mouse ascites fluid
Application WB, IP, IS
Image 1
BL-21 bacteria lysate
1:5000 (0.2 ug/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein.
 

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