TBP Antibody (OADC00242)

• Product Number: OADC00242
• Name: TBP Antibody (OADC00242)
• Isotype: IgG1
• Conjugation: Unconjugated
• NCBI Gene Id: 6908;
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 8 mg/mL
• Gene Full Name: TATA-box binding protein
• Alias Symbols: HDL4, GTF2D, SCA17, TFIID, GTF2D1
• Product Format: Liquid. Provided in PBS containing 0.05% azide; purified by ammonium sulphate precipitation followed by dialysis.
• Description of Target: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Specificity: Monoclonal antibody raised in mouse against the amino-terminal domain of human TBP (TATA box binding protein).
• Application Info: CHIP: 4 - 5 ug/IP
• Uniprot ID: P20226
• Protein Name: TATA-box-binding protein
• Protein Accession #: NP_001165556.1
• Purification: Protein A/G purified
• Nucleotide Accession #: NM_001172085.1
• Preservative: 0.05% Sodium Azide
• Gene Symbol: TBP
• Predicted Species Reactivity: Human
• Application: CHIP
• Predicted Homology Based on Immunogen Sequence: Mouse
• Image 1: HelaS3 cells
  
  ChIP-seq results obtained with the Aviva monoclonal antibody directed against TBP r. ChIP was performed with 5 ug of the Aviva antibody against TBP on sheared chromatin from 1 million HeLaS3 cells. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and for a region 1 kb upstream of the GAPDH promoter and the coding region of the inactive MB gene, used as negative control targets. The IP'd DNA was subsequently analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure shows the peak distribution in 50 kb regions surrounding the GAPDH, c-fos, ACTB and MCL1 genes (figure 2B, C, D and E, respectively). These results clearly show a localisation of TBP at the promoters of actively transcribed genes.
• Image 2: U2OS cells
  
  ChIP results obtained with the Aviva monoclonal antibody against TBP. ChIP assays were performed using U2OS cells, the Aviva antibody directed against TBP and optimized primer sets for qPCR. Sheared chromatin from 1x10e6 cells and 4 ug of antibody were used per ChIP experiment. QPCR was performed with primers for the promoter of the c-fos and GAPDH genes, a region 0.5 and 1 kb upstream of the GAPDH promoter, respectively, and for exon 2 of the myoglobin gene as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of both promoters by TBP.