RFXAP Antibody (OADC00212)

• Product Number: OADC00212
• Name: RFXAP Antibody (OADC00212)
• Conjugation: Unconjugated
• NCBI Gene Id: 5994;
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Gene Full Name: regulatory factor X associated protein
• Alias Symbols: Regulatory factor X-associated protein, RFX-associated protein, RFX DNA-binding complex 36 kDa subunit
• Product Format: Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300.
• Description of Target: Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Specificity: Polyclonal antibody raised in rabbit against RFXAP (Regulatory factor X-associated protein), using the recombinant protein.
• Application Info: CHIP: 5-7 ug/IP
• Uniprot ID: O00287
• Protein Name: regulatory factor X-associated protein
• Protein Accession #: NP_000529.1
• Purification: Affinity purified
• Nucleotide Accession #: NM_000538.3
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300.
• Gene Symbol: RFXAP
• Predicted Species Reactivity: Human
• Application: CHIP
• Image 1: NALM cells
  
  ChIP results obtained with the Aviva antibody directed against RFXAPr. ChIP assays were performed using NALM cells (a cell line derived from human pre-B leukemia), the Aviva antibody against RFXAP and optimized primer sets for PCR. Sheared chromatin from 2 million cells and respectively 5 and 7 ug of antibody were used per ChIP experiment. An anti-flag antibody (lane 2) was used as negative IP control. Figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel).