RARA Antibody (OADC00209)

Data Sheet
 
Product Number OADC00209
Product Page www.avivasysbio.com/rara-antibody-oadc00209.html
Name RARA Antibody (OADC00209)
Conjugation Unconjugated
NCBI Gene Id 5914;
Host Rabbit
Clonality Polyclonal
Concentration Undetermined, ascites
Gene Full Name retinoic acid receptor alpha
Alias Symbols RAR, NR1B1
Product Format Liquid. Whole antiserum from rabbit containing 0.05% azide.
Description of Target This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
Reconstitution and Storage Store at -20C. Avoid repeated freeze/thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RARA (OADC00209) antibody
Specificity Polyclonal antibody raised in rabbit against human RARA (Retinoic Acid Receptor alpha) using two KLH-conjugated synthetic peptides containing sequences from the C-terminal region of the protein.
Application Info CHIP/CHIP-Seq: 4 ug/CHIP
ELISA: 1:50
Western Blotting: 1:750
Uniprot ID P10276
Protein Name retinoic acid receptor alpha
Protein Accession # NP_000955.1
Purification Whole antiserum
Nucleotide Accession # NM_000964.3
Preservative 0.05% Sodium Azide
Gene Symbol RARA
Predicted Species Reactivity Human
Application CHIP, WB
Image 1
NB4 cells
ChIP results obtained with the Aviva antibody directed against H3K36me3r. ChIP assays were performed using NB4 cells, the Aviva antibody against RARA and optimized primer pairs for qPCR. Sheared chromatin from 6 million cells and 4 ul of antibody were used per ChIP experiment. QPCR was performed using primers specific for the TGM2, HMHA1, PRAM1 and H2B genes. Figure shows the relative occupancy, calculated as the ratio + control/background for which the second exon of the MB gene was used.
Image 2
Human reference genome (hg18)
ChIP-seq results obtained with the Aviva antibody directed against RARAr. ChIP was performed as described above and the IP'd DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 32 bp tags were aligned to the human reference genome (hg18) using the ELAND algorithm. Figure shows the results of the complete chromosome 19 and two 50 kb region surrounding the HMHA1 and PRAM1 genes, respectively.
Image 3
Human RARA
Determination of the antibody titer. To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Aviva antibody directed against human RARA. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:2,400.
Image 4
Human embryonic kidney cells (293T)
Western blot analysis using the Aviva antibody directed against RARAr. Human embryonic kidney cells (293T) were transfected with a RARA construct (lane 2) or with a negative control construct (lane 1) and analysed by Western blot using the Aviva antibody against RARA, diluted 1:750 in BSA/PBS- Tween. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.
 

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