Product Number |
OADC00148 |
Product Page |
www.avivasysbio.com/his-tag-antibody-oadc00148.html |
Name |
His-Tag Antibody (OADC00148) |
Isotype |
IgG2b |
Conjugation |
Unconjugated |
Host |
Mouse |
Clonality |
Monoclonal |
Concentration |
2.1 mg/mL |
Product Format |
Liquid. Provided in PBS (pH 7.2). |
Description of Target |
Monoclonal antibody raised in mouse against the 6xHis tag (amino acid sequence HHHHHH) using a KLH-conjugated synthetic peptide. The antibody recognizes the Histidine epitope which is one of the most popular protein tags. The Diagenode His tag antibody is extremely specific and allows unambiguous identification and quantitative analysis of His-tagged proteins. |
Reconstitution and Storage |
Store at -20C. Avoid repeated freeze/thaw cycles. |
Datasheets/Manuals |
Printable datasheet for His-Tag (OADC00148) antibody |
Specificity |
Monoclonal antibody raised in mouse against the 6xHis tag (amino acid sequence HHHHHH) using a KLH-conjugated synthetic peptide. The antibody recognizes the Histidine epitope which is one of the most popular protein tags. The Diagenode His tag antibody is extremely specific and allows unambiguous identification and quantitative analysis of His-tagged proteins. |
Application Info |
Western Blotting: 1:1,000 - 1:3,000 IP/CHIP 1:150 - 1:200 IS 1:500 - 1:1,000 |
Purification |
Protein A purified |
Predicted Species Reactivity |
Specific for His-tagged fusion proteins expressed in bacteria |
Application |
WB, IP, CHIP, IS |
Predicted Homology Based on Immunogen Sequence |
Mammalian cells |
Image 1 | Bacterial extracts
| Western blot analysis using the Aviva monoclonal antibody against the His protein tag. Bacterial extracts containing a His fusion protein were analyzed by Western blot using the Aviva monoclonal antibody directed against His. The antibody was used at a dilution of 1:1,000. The MW marker (in kDa) is shown on the left. The protein of interest (expected size of 27 kDa) is detected as a single band showing the high specificity of the His monoclonal antibody. |
| Image 2 | HEK293 cells
| Western blot analysis using the Aviva monoclonal antibody against the His protein tag. Western blot was performed on whole cell extracts from HEK293 cells transfected with a His-tagged expression vector using the Aviva monoclonal antibody directed against His. The antibody was used at a dilution of 1:1,000. Figure shows the results for cells transfected with a HA-tagged protein (lane 1) and for untransfected cells, used as a negative control (lane 2). The MW marker (in kDa) is shown on the left. A single band is clearly visible in lane 1, but absent in lane 2 showing the high specificity of the His antibody. |
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