Product Number |
OADC00091 |
Product Page |
www.avivasysbio.com/histone-h3-antibody-tri-methyl-lys36-oadc00091.html |
Name |
Histone H3 Antibody (Tri-Methyl-Lys36) (OADC00091) |
Conjugation |
Unconjugated |
NCBI Gene Id |
8290; 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968 |
Host |
Mouse |
Clonality |
Monoclonal |
Concentration |
1 mg/mL |
Gene Full Name |
histone cluster 3, H3 |
Alias Symbols |
H3t, H3.4, H3/g, H3FT, HIST3H3 |
Product Format |
Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300. |
Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Reconstitution and Storage |
Store at -20C. Avoid repeated freeze/thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-HIST3H3 (OADC00091) antibody |
Specificity |
Monoclonal antibody raised in mouse against histone H3 trimethylated at lysine 36 (H3K36me3), using a KLH-conjugated synthetic peptide. |
Application Info |
CHIP: 1-5 ug/CHIP ELISA: 1:3,000 Dot Blotting: 1:10,000 Western Blotting: 1:1,000 - 1:2,000 IF: 1:500, |
Uniprot ID |
P68431 |
Protein Name |
histone H3.1t |
Protein Accession # |
NP_003520.1 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_003529.2 |
Target Post-Translational Modification |
Tri-Methyl-Lys36 |
Preservative |
0.05% Sodium Azide and 0.05% ProClin 300. |
Gene Symbol |
HIST3H3 |
Predicted Species Reactivity |
Human |
Application |
CHIP, DB, WB, IF |
Image 1 | H3K36me3
| Cross reactivity of the Aviva monoclonal antibody directed against H3K36me3. To test the specificity an ELISA was performed using a serial dilution of the Aviva monoclonal antibody against H3K36me3. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest. |
| Image 2 | Hela cells
| Immunofluorescence using the Aviva monoclonal antibody directed against H3K36me3. HeLa cells were stained with the Aviva antibody against H3K36me3 and with DAPI. Cells were fixed with methanol and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right. |
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