| Product Number |
OADC00089 |
| Product Page |
www.avivasysbio.com/histone-h3-antibody-di-methyl-lys36-oadc00089.html |
| Name |
Histone H3 Antibody (Di-Methyl-Lys36) (OADC00089) |
| Isotype |
Isotype: IgG1* |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
8290; 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968 |
| Host |
Mouse |
| Clonality |
Monoclonal |
| Concentration |
1 mg/mL |
| Gene Full Name |
histone cluster 3, H3 |
| Alias Symbols |
H3t, H3.4, H3/g, H3FT, HIST3H3 |
| Product Format |
Liquid. Provided in PBS containing 0.05% azide. |
| Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Reconstitution and Storage |
Store at -20C. Avoid repeated freeze/thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-HIST3H3 (OADC00089) antibody |
| Specificity |
Monoclonal antibody raised in mouse against histone H3 dimethylated at lysine 36 (H3K36me2), using a KLH-conjugated synthetic peptide. |
| Application Info |
CHIP: 1 - 5 ug/CHIP
ELISA: 1:3,000
Dot Blotting: 1:10,000
Western Blotting: 1:1,000 - 1:2,000 |
| Uniprot ID |
P68431 |
| Protein Name |
histone H3.1t |
| Protein Accession # |
NP_003520.1 |
| Purification |
Protein A purified |
| Nucleotide Accession # |
NM_003529.2 |
| Target Post-Translational Modification |
Di-Methyl-Lys36 |
| Preservative |
0.05% Sodium Azide |
| Gene Symbol |
HIST3H3 |
| Predicted Species Reactivity |
Human |
| Application |
CHIP, DB, WB |
| Image 1 | H3K36me2
 | Cross reactivity of the Aviva monoclonal antibody directed against H3K36me2. To test the specificity an ELISA was performed using a serial dilution of the Aviva monoclonal antibody against H3K36me2. The wells were coated with peptides containing the unmodified H3K36 region as well as the mono-, di- and trimethylated H3K36 and the trimethylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest. |
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