Histone H3 Antibody (Acetyl-Lys27) (OADC00079)

• Product Number: OADC00079
• Name: Histone H3 Antibody (Acetyl-Lys27) (OADC00079)
• Isotype: IgG1*
• Conjugation: Unconjugated
• NCBI Gene Id: 8290; 8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
• Host: Mouse
• Clonality: Monoclonal
• Concentration: 1 mg/mL
• Gene Full Name: histone cluster 3, H3
• Alias Symbols: H3t, H3.4, H3/g, H3FT, HIST3H3
• Product Format: Liquid. Provided in PBS containing 0.05% azide and 0.05% ProClin 300.
• Description of Target: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Specificity: Monoclonal antibody raised in mouse against histone H3 acetylated at lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide.
• Application Info: CHIP: 1-5 ug/CHIP
  ELISA: 1:3,000
  Western Blotting: 1:1,000, 1:2,000
  IF: 1:500
• Uniprot ID: P68431
• Protein Name: histone H3.1t
• Protein Accession #: NP_003520.1
• Purification: Protein A purified
• Nucleotide Accession #: NM_003529.2
• Target Post-Translational Modification: Acetyl-Lys27
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300.
• Gene Symbol: HIST3H3
• Predicted Species Reactivity: Human
• Application: CHIP, WB, IF
• Image 1: H3K27ac
  
  Cross reactivity of the Aviva monoclonal antibody directed against H3K27ac. To test the specificity an ELISA was performed using a serial dilution of the Aviva monoclonal antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Figure shows a high specificity of the antibody for the peptide containing the modification of interest.
• Image 2: Hela cells
  
  Immunofluorescence using the Aviva monoclonal antibody directed against H3K27ac. HeLa cells were stained with the Aviva antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.