| Product Number |
OACD08298 |
| Product Page |
www.avivasysbio.com/xpo1-antibody-oacd08298.html |
| Name |
XPO1 Antibody (OACD08298) |
| Molecular Weight |
123 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
7514 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
exportin 1 |
| Alias Symbols |
chromosome region maintenance 1 homolog;chromosome region maintenance 1 protein homolog;CRM1;CRM-1;emb;exp1;exportin 1 (CRM1 homolog, yeast);exportin-1;exportin-1 (required for chromosome region maintenance). |
| Product Format |
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
| Description of Target |
Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase RAN in its active GTP-bound form (Ran-GTP). Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap. Several viruses, among them HIV-1, HTLV-1 and influenza A use it to export their unspliced or incompletely spliced RNAs out of the nucleus. Interacts with, and mediates the nuclear export of HIV-1 Rev and HTLV-1 Rex proteins. Involved in HTLV-1 Rex multimerization. |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for XPO1 Antibody (OACD08298) |
| Specificity |
The antibody is a rabbit polyclonal antibody raised against XPO1. It has been selected for its ability to recognize XPO1 in immunohistochemical staining and western blotting. |
| Immunogen |
Range: Thr917 - Asp1071 |
| Formulation |
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Uniprot ID |
O14980 |
| Protein Name |
Exportin-1 |
| Protein Accession # |
NP_003391.1 |
| Purification |
Affinity Purified; Protein A Purified |
| Application Data |
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
Optimal working dilutions must be determined by end user. |
| Nucleotide Accession # |
NM_003400 |
| Preservative |
0.02% NaN3 |
| Gene Symbol |
XPO1 |
| Predicted Species Reactivity |
Human|Porcine |
| Application |
Immunohistochemistry|Western blot |
| Image 1 | Exportin-1
 | Western Blot with Human Recombinant Exportin-1 |
| | Image 2 | WT and KO HeLa Cell Lysate
 | Verification of specificity by knock-out - Lane 1: Wild-type Hela cell lysate, Lane 2: XPo1 knockout Hela cell lysate, Predicted MW: 123kd Observed MW: 110kd, Primary Ab: 1 ug/mL XPO1 Antibody (OACD08298), Secondary Ab: 0.2 ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody |
| | Image 3 | Hela cells
 | FITC staining on formalin fixed paraffin-embedded Hela cells |
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