| Product Number |
OACD01856 |
| Product Page |
www.avivasysbio.com/atp5b-antibody-oacd01856.html |
| Name |
ATP5B Antibody (OACD01856) |
| Molecular Weight |
56 kDa |
| Isotype |
IgG |
| NCBI Gene Id |
171374 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.43 mg/ml |
| Gene Full Name |
ATP synthase F1 subunit beta |
| Alias Symbols |
ATP synthase F1 subunit beta;ATP synthase subunit beta, mitochondrial;ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit;ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide;Atp5b;mitochondrial ATP synthase beta subunit. |
| Product Format |
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
| Description of Target |
Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for ATP5B Antibody (OACD01856) |
| Specificity |
The antibody is a rabbit polyclonal antibody raised against ATP5b. It has been selected for its ability to recognize ATP5b in immunohistochemical staining and western blotting. |
| Immunogen |
Range: Ala47 - Ser529 |
| Formulation |
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
| Uniprot ID |
P10719 |
| Protein Name |
ATP synthase subunit beta, mitochondrial |
| Protein Accession # |
NP_599191.1 |
| Purification |
Affinity Purified; Protein A Purified |
| Application Data |
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
Optimal working dilutions must be determined by end user. |
| Nucleotide Accession # |
NM_134364 |
| Preservative |
0.02% NaN3 |
| Gene Symbol |
Atp5f1b |
| Predicted Species Reactivity |
Human|Mouse|Rat |
| Application |
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Image 1 | ATP synthase subunit beta, mitochondrial
 | Western Blot with Rat Recombinant ATP synthase subunit beta, mitochondrial |
| | Image 2 | Liver tissue
 | DAB staining on formalin fixed paraffin-embedded liver tissue |
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