Product Number |
OACD01026 |
Product Page |
www.avivasysbio.com/dapk3-antibody-oacd01026.html |
Name |
DAPK3 Antibody (OACD01026) |
Molecular Weight |
51 kDa |
Isotype |
IgG |
NCBI Gene Id |
13144 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
death-associated protein kinase 3 |
Alias Symbols |
DAP kinase 3;DAP-like kinase;death-associated kinase 3;death-associated protein kinase 3;dlk;MYPT1 kinase;ZIPK;ZIP-kinase. |
Product Format |
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol. |
Description of Target |
Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in formation of promyelocytic leukemia protein nuclear body (PML-NB). Involved in apoptosis involving PAWR which mediates cytoplasmic relocation; in vitro phosphorylates PAWR (By similarity). Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton such as in regulation of cell polarity and cell migration. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2; disrupts the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates STAT3 and enhances its transcriptional activity. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis (By similarity). Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for DAPK3 Antibody (OACD01026) |
Specificity |
The antibody is a rabbit polyclonal antibody raised against DAPK3. It has been selected for its ability to recognize DAPK3 in immunohistochemical staining and western blotting. |
Immunogen |
Range: Met1 - Arg448 |
Formulation |
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
Uniprot ID |
O54784 |
Protein Name |
Death-associated protein kinase 3 |
Protein Accession # |
NP_001177402.1 |
Purification |
Affinity Purified; Protein A Purified |
Application Data |
Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
Nucleotide Accession # |
NM_001190473 |
Preservative |
0.02% NaN3 |
Gene Symbol |
Dapk3 |
Predicted Species Reactivity |
Mouse|Rat |
Application |
Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
Image 1 | Death-associated protein kinase 3
| Western Blot with Mouse Recombinant Death-associated protein kinase 3 |
|
|