| Product Number |
OACA10075 |
| Product Page |
www.avivasysbio.com/aak1-antibody-oaca10075.html |
| Name |
AAK1 Antibody (OACA10075) |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
22848 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Purity |
>95% |
| Gene Full Name |
AP2 associated kinase 1 |
| Alias Symbols |
adaptor-associated kinase 1;AP2-associated protein kinase 1. |
| Product Format |
Liquid |
| Description of Target |
Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. |
| Reconstitution and Storage |
-20°C or -80°C |
| Datasheets/Manuals |
Printable datasheet for AAK1 Antibody (OACA10075) (OACA10075) |
| Immunogen |
Recombinant Human AP2-associated protein kinase 1 protein (274-465AA) |
| Formulation |
50% Glycerol, 0.01M PBS, PH 7.4 |
| Uniprot ID |
Q2M2I8 |
| Protein Name |
AP2-associated protein kinase 1 |
| Protein Accession # |
NP_055726 |
| Purification |
Protein G Purified |
| Nucleotide Accession # |
NM_014911 |
| Preservative |
0.03% Proclin 300 |
| Gene Symbol |
AAK1 |
| Predicted Species Reactivity |
Human|Mouse |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot |
| Image 1 | Mouse kidney tissue
 | Western Blot
Positive WB detected in: Mouse kidney tissue
All lanes: AAK1 antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/5,0000 dilution
Predicted band size: 104,94 kDa
Observed band size: 104 kDa
|
| | Image 2 | Human brain tissue
 | Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100 |
| | Image 3 | HepG2 cells
 | Immunofluorescent analysis of HepG2 cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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