| Product Number |
OACA09998 |
| Product Page |
www.avivasysbio.com/dapk3-antibody-oaca09998.html |
| Name |
DAPK3 Antibody (OACA09998) |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
1613 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Purity |
>95% |
| Gene Full Name |
death associated protein kinase 3 |
| Alias Symbols |
DAP kinase 3;DAP-like kinase;death-associated protein kinase 3;DLK;MYPT1 kinase;ZIP;ZIPK;ZIP-kinase;zipper-interacting protein kinase. |
| Product Format |
Liquid |
| Description of Target |
Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor. |
| Reconstitution and Storage |
-20°C or -80°C |
| Datasheets/Manuals |
Printable datasheet for DAPK3 Antibody (OACA09998) (OACA09998) |
| Immunogen |
Recombinant Human Death-associated protein kinase 3 protein (283-401AA) |
| Formulation |
50% Glycerol, 0.01M PBS, PH 7.4 |
| Uniprot ID |
O43293 |
| Protein Name |
Death-associated protein kinase 3 |
| Protein Accession # |
NP_001339 |
| Purification |
Protein G Purified |
| Nucleotide Accession # |
NM_001348 |
| Preservative |
0.03% Proclin 300 |
| Gene Symbol |
DAPK3 |
| Predicted Species Reactivity |
Human |
| Application |
Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot |
| Image 1 | HepG2 whole cell lysate, Mouse brain tissue
 | Western Blot
Positive WB detected in: HepG2 whole cell lysate,Mouse brain tissue
All lanes: DAPK3 antibody at 3.4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/5,0000 dilution
Predicted band size: 53,38 kDa
Observed band size: 53 kDa
|
| | Image 2 | Hela cells
 | Immunofluorescent analysis of Hela cells at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) |
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