FAP Antibody (OACA06849)

Data Sheet
 
Product Number OACA06849
Product Page www.avivasysbio.com/fap-antibody-oaca06849.html
Name FAP Antibody (OACA06849)
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 2191
Host Rabbit
Clonality Polyclonal
Concentration Varies by lot. See vial for concentration.
Gene Full Name fibroblast activation protein alpha
Alias Symbols 170 kDa melanoma membrane-bound gelatinase;dipeptidyl peptidase FAP;DPPIV;FAPA;FAPalpha;Fibroblast activation protein alpha;gelatine degradation protease FAP;integral membrane serine protease;post-proline cleaving enzyme;prolyl endopeptidase FAP;seprase;Serine integral membrane protease;SIMP;surface-expressed protease.
Product Format Liquid
Reference [1]Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha."Aertgeerts K., Levin I., Shi L., Snell G.P., Jennings A., Prasad G.S., Zhang Y., Kraus M.L., Salakian S., Sridhar V., Wijnands R., Tennant M.G. J. Biol. Chem. 280:19441-19444(2005). [2]A rare variant in human fibroblast activation protein associated with ER stress, loss of enzymatic function and loss of cell surface localisation." Osborne B., Yao T.W., Wang X.M., Chen Y., Kotan L.D., Nadvi N.A., Herdem M., McCaughan G.W., Allen J.D., Yu D.M., Topaloglu A.K., Gorrell M.D.Biochim. Biophys. Acta 1844:1248-1259(2014). [3]Quantitation of fibroblast activation protein (FAP)-specific protease activity in mouse, baboon and human fluids and organs."Keane F.M., Yao T.W., Seelk S., Gall M.G., Chowdhury S., Poplawski S.E., Lai J.H., Li Y., Wu W., Farrell P., Vieira de Ribeiro A.J., Osborne B., Yu D.M., Seth D., Rahman K., Haber P., Topaloglu A.K., Wang C. Gorrell M.D.FEBS Open Bio 4:43-54(2013).
Description of Target Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2 (PubMed:14751930, PubMed:16223769, PubMed:16480718, PubMed:16410248, PubMed:17381073, PubMed:18095711, PubMed:21288888, PubMed:24371721). Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein (PubMed:9065413, PubMed:2172980, PubMed:7923219, PubMed:10347120, PubMed:10455171, PubMed:12376466, PubMed:16223769, PubMed:16651416, PubMed:18095711). Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro (PubMed:10347120, PubMed:10593948, PubMed:16175601, PubMed:16223769, PubMed:16651416, PubMed:16410248, PubMed:17381073, PubMed:21314817, PubMed:24371721, PubMed:24717288). Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.
Reconstitution and Storage -20°C or -80°C
Datasheets/Manuals Printable datasheet for FAP Antibody (OACA06849) (OACA06849)
Application Info IHC: 1:20~200
Immunogen Recombinant Human Prolyl endopeptidase FAP protein (26-280AA)
Uniprot ID Q12884
Protein Name Prolyl endopeptidase FAP
Protein Accession # NP_001278736
Purification Affinity Purified
Nucleotide Accession # NM_001291807
Gene Symbol FAP
Predicted Species Reactivity Human
Application Enzyme-linked immunosorbent assay|Immunohistochemistry
Image 1
Human skin
Immunohistochemistry of paraffin-embedded human skin at dilution 1:100
Image 2
Human small intestine
Immunohistochemistry of paraffin-embedded human small intestine at dilution 1:100
 

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