Product Number |
OACA06783 |
Product Page |
www.avivasysbio.com/nprl2-antibody-oaca06783.html |
Name |
NPRL2 Antibody (OACA06783) |
Isotype |
IgG |
Conjugation |
Unconjugated |
NCBI Gene Id |
10641 |
Host |
Rabbit |
Clonality |
Polyclonal |
Gene Full Name |
NPR2 like, GATOR1 complex subunit |
Alias Symbols |
2810446G01Rik;FFEVF2;G21 protein;GATOR complex protein NPRL2;gene 21 protein;homologous to yeast nitrogen permease (candidate tumor suppressor);nitrogen permease regulator 2-like protein;NPR2;NPR2L;NPR2-like protein;tumor suppressor candidate 4;TUSC4. |
Product Format |
Liquid |
Reference |
[1]A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1."Bar-Peled L., Chantranupong L., Cherniack A.D., Chen W.W., Ottina K.A., Grabiner B.C., Spear E.D., Carter S.L., Meyerson M., Sabatini D.M.Science 340:1100-1106(2013). [2]A genome-wide screen for regulators of TORC1 in response to amino acid starvation reveals a conserved Npr2/3 complex."Neklesa T.K., Davis R.W.PLoS Genet. 5:E1000515-E1000515(2009). [3]TUSC4/NPRL2, a novel PDK1-interacting protein, inhibits PDK1 tyrosine phosphorylation and its downstream signaling."Kurata A., Katayama R., Watanabe T., Tsuruo T., Fujita N.Cancer Sci. 99:1827-1834(2008) |
Description of Target |
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway. |
Reconstitution and Storage |
-20°C or -80°C |
Datasheets/Manuals |
Printable datasheet for NPRL2 Antibody (OACA06783) (OACA06783) |
Immunogen |
Recombinant Human Nitrogen permease regulator 2-like protein (1-220AA) |
Formulation |
PBS with, 50% glycerol, pH7.3. |
Uniprot ID |
Q8WTW4 |
Protein Name |
GATOR complex protein NPRL2 |
Protein Accession # |
NP_006536 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_006545 |
Preservative |
0.02% sodium azide |
Gene Symbol |
NPRL2 |
Predicted Species Reactivity |
Human|Mouse |
Application |
Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot |
Image 1 | Mouse skeletal muscle tissue
| All lanes: Nitrogen permease regulator 2-like protein antibody at 7ug/ml+mouse skeletal muscle tissue
Secondary
Goat polyclonal to rabbit at 1/1,0000 dilution
Predicted band size: 44,24 kDa
Observed band size: 44 kDa
|
| Image 2 | Human lung cancer
| Immunohistochemistry of paraffin-embedded human lung cancer at dilution 1:100 |
| Image 3 | Human rectum
| Immunohistochemistry of paraffin-embedded human rectum at dilution 1:100 |
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