SMARCA2 Antibody (OACA02435)

• Product Number: OACA02435
• Name: SMARCA2 Antibody (OACA02435)
• Protein Size (# AA): 1590 amino acids
• Molecular Weight: 181 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 6595
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Alias Symbols: ATP-dependent helicase SMARCA2;BAF190;BAF190B;BIS;brahma homolog;BRG1-associated factor 190B;BRM;global transcription activator homologous sequence;hBRM;hSNF2a;NCBRS;probable global transcription activator SNF2L2;protein brahma homolog;SNF2;SNF2/SWI2-like protein 2;SNF2-alpha;SNF2L2;SNF2LA;Sth1p;sucrose nonfermenting 2-like protein 2;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a2;SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2;SWI2.
• Product Format: Liquid
• Reference: [1] A quantitative atlas of mitotic phosphorylation. Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767 (2008).
  [2] Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography. Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J. Proteomics 8:1346-1361 (2008).
  [3] Initial characterization of the human central proteome. Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17 (2011).
• Description of Target: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity).
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Probable global transcription activator SNF2L2 protein (700-1216AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: P51531
• Protein Name: Probable global transcription activator SNF2L2
• Protein Accession #: NP_001276325.1
• Purification: Protein G Purified
• Nucleotide Accession #: NM_001289396.1
• Gene Symbol: SMARCA2
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-BRM or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 pS2 promoter.
• Image 2: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis using OACA02435 at dilution 1: 100