RELA Antibody (OACA02434)

• Product Number: OACA02434
• Name: RELA Antibody (OACA02434)
• Protein Size (# AA): 551 amino acids
• Molecular Weight: 60 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 5970
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: RELA proto-oncogene, NF-kB subunit
• Alias Symbols: CMCU;NF-kappa-B p65delta3;NF-kappa-B transcription factor p65;NFKB3;nuclear factor NF-kappa-B p65 subunit;nuclear factor of kappa light polypeptide gene enhancer in B-cells 3;p65;transcription factor p65;v-rel avian reticuloendotheliosis viral oncogene homolog A.
• Product Format: Liquid
• Reference: [1] Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kappaB response. Saccani S., Marazzi I., Beg A.A., Natoli G.J. Exp. Med. 200:107-113 (2004).
  [2] COMMD proteins, a novel family of structural and functional homologs of MURR1. Burstein E., Hoberg J.E., Wilkinson A.S., Rumble J.M., Csomos R.A., Komarck C.M., Maine G.N., Wilkinson J.C., Mayo M.W., Duckett C.S.J. Biol. Chem. 280:22222-22232 (2005).
  [3] Inducible phosphorylation of NF-kappa B p65 at serine 468 by T cell costimulation is mediated by IKK epsilon. Mattioli I., Geng H., Sebald A., Hodel M., Bucher C., Kracht M., Schmitz M.L. J. Biol. Chem. 281:6175-6183 (2006).
• Description of Target: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Transcription factor p65 protein (1-210AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: Q04206
• Protein Name: Transcription factor p65
• Protein Accession #: NP_001138610.1
• Purification: Protein G Purified
• Nucleotide Accession #: NM_001145138.1
• Gene Symbol: RELA
• Predicted Species Reactivity: Human|Mouse
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.2*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-NF-kB p65 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the IkB? promoter.
• Image 2: Human Placenta
  
  Immunohistochemistry of paraffin-embedded human placenta using at dilution of 1: 100