POLR3A Antibody (OACA02433)

• Product Number: OACA02433
• Name: POLR3A Antibody (OACA02433)
• Protein Size (# AA): 1390 amino acids
• Molecular Weight: 156 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 11128
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: RNA polymerase III subunit A
• Alias Symbols: ADDH;C160;DNA-directed RNA polymerase III largest subunit;DNA-directed RNA polymerase III subunit A;DNA-directed RNA polymerase III subunit RPC1;HLD7;hRPC155;polymerase (RNA) III (DNA directed) polypeptide A, 155kDa;polymerase (RNA) III subunit A;RNA polymerase III 155 kDa subunit;RNA polymerase III subunit C1;RNA polymerase III subunit C160;RNA polymerase III subunit RPC155-D;RPC1;RPC155;WDRTS.
• Product Format: Liquid
• Reference: [1] Novel compound heterozygous mutations of POLR3A revealed by whole-exome sequencing in a patient with hypomyelination. Shimojima K., Shimada S., Tamasaki A., Akaboshi S., Komoike Y., Saito A., Furukawa T., Yamamoto T. Brain Dev. 36:315-321 (2014).
  [2] Mutations in POLR3A and POLR3B are a major cause of hypomyelinating leukodystrophies with or without dental abnormalities and/or hypogonadotropic hypogonadism. Daoud H., Tetreault M., Gibson W., Guerrero K., Cohen A., Gburek-Augustat J., Synofzik M., Brais B., Stevens C.A., Sanchez-Carpintero R., Goizet C., Naidu S., Vanderver A., Bernard G. J. Med. Genet. 50:194-197 (2013).
  [3] Mutations in POLR3A and POLR3B encoding RNA Polymerase III subunits cause an autosomal-recessive hypomyelinating leukoencephalopathy. Saitsu H., Osaka H., Sasaki M., Takanashi J., Hamada K., Yamashita A., Shibayama H., Shiina M., Kondo Y., Nishiyama K., Tsurusaki Y., Miyake N., Doi H., Ogata K., Inoue K., Matsumoto N. Am. J. Hum. Genet. 89:644-651 (2011).
• Description of Target: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity). Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human DNA-directed RNA polymerase III subunit RPC1 protein (392-632AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: O14802
• Protein Name: DNA-directed RNA polymerase III subunit RPC1
• Protein Accession #: NP_008986.2
• Purification: Protein G Purified
• Nucleotide Accession #: NM_007055.3
• Gene Symbol: POLR3A
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293T
  
  293T (1.6*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-POLR3A or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the tRNA-Leu Anti-Codon (TAG) promoter.
• Image 2: Human Small Intestine
  
  Immunohistochemistry of paraffin-embedded human small intestine tissue usingOACA02433 at dilution 1: 100
• Image 3: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis using OACA02433 at dilution 1: 100