ESR1 Antibody (OACA02432)

Data Sheet
 
Product Number OACA02432
Product Page www.avivasysbio.com/esr1-antibody-oaca02432.html
Name ESR1 Antibody (OACA02432)
Protein Size (# AA) 595 amino acids
Molecular Weight 66 kDa
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 2099
Host Rabbit
Clonality Polyclonal
Gene Full Name estrogen receptor 1
Alias Symbols E2 receptor alpha;ER;Era;ER-alpha;ESR;ESRA;estradiol receptor;estrogen nuclear receptor alpha;estrogen receptor;estrogen receptor alpha E1-E2-1-2;estrogen receptor alpha E1-N2-E2-1-2;ESTRR;NR3A1;nuclear receptor subfamily 3 group A member 1;oestrogen receptor alpha.
Product Format Liquid
Description of Target Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1.
Reconstitution and Storage -20°C or -80°C
Datasheets/Manuals Printable datasheet for ESR1 Antibody (OACA02432) (OACA02432)
Specificity Human
Immunogen Recombinant Human Estrogen receptor protein (10-591AA)
Formulation Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
Uniprot ID P03372
Protein Name Estrogen receptor
Protein Accession # NP_000116.2
Purification Protein G Purified
Nucleotide Accession # NM_000125.3
Gene Symbol ESR1
Predicted Species Reactivity Human|Rat
Application Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
Predicted Homology Based on Immunogen Sequence Human
Image 1
MCF-7
MCF7 (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter.
Image 2
Human Breast Cancer
Immunohistochemistry of paraffin-embedded human breast cancer using OACA02432 at dilution 1: 100
 

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