Product Number |
OACA02432 |
Product Page |
www.avivasysbio.com/esr1-antibody-oaca02432.html |
Name |
ESR1 Antibody (OACA02432) |
Protein Size (# AA) |
595 amino acids |
Molecular Weight |
66 kDa |
Isotype |
IgG |
Conjugation |
Unconjugated |
NCBI Gene Id |
2099 |
Host |
Rabbit |
Clonality |
Polyclonal |
Gene Full Name |
estrogen receptor 1 |
Alias Symbols |
E2 receptor alpha;ER;Era;ER-alpha;ESR;ESRA;estradiol receptor;estrogen nuclear receptor alpha;estrogen receptor;estrogen receptor alpha E1-E2-1-2;estrogen receptor alpha E1-N2-E2-1-2;ESTRR;NR3A1;nuclear receptor subfamily 3 group A member 1;oestrogen receptor alpha. |
Product Format |
Liquid |
Description of Target |
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. |
Reconstitution and Storage |
-20°C or -80°C |
Datasheets/Manuals |
Printable datasheet for ESR1 Antibody (OACA02432) (OACA02432) |
Specificity |
Human |
Immunogen |
Recombinant Human Estrogen receptor protein (10-591AA) |
Formulation |
Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300 |
Uniprot ID |
P03372 |
Protein Name |
Estrogen receptor |
Protein Accession # |
NP_000116.2 |
Purification |
Protein G Purified |
Nucleotide Accession # |
NM_000125.3 |
Gene Symbol |
ESR1 |
Predicted Species Reactivity |
Human|Rat |
Application |
Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot |
Predicted Homology Based on Immunogen Sequence |
Human |
Image 1 | MCF-7
| MCF7 (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-ESR1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ESR1 promoter. |
| Image 2 | Human Breast Cancer
| Immunohistochemistry of paraffin-embedded human breast cancer using OACA02432 at dilution 1: 100 |
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