| Product Number |
OACA02431 |
| Product Page |
www.avivasysbio.com/chd1-antibody-oaca02431.html |
| Name |
CHD1 Antibody (OACA02431) |
| Protein Size (# AA) |
1710 amino acids |
| Molecular Weight |
197 kDa |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
1105 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Varies by lot. See vial for concentration. |
| Gene Full Name |
chromodomain helicase DNA binding protein 1 |
| Alias Symbols |
ATP-dependent helicase CHD1;CHD-1;chromodomain-helicase-DNA-binding protein 1;PILBOS. |
| Product Format |
Liquid |
| Reference |
[1] The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127 (2004).
[2] Human but not yeast CHD1 binds directly and selectively to histone H3 methylated at lysine 4 via its tandem chromodomains. Sims R.J. III, Chen C.-F., Santos-Rosa H., Kouzarides T., Patel S.S., Reinberg D.J. Biol. Chem. 280:41789-41792 (2005). |
| Description of Target |
ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Is also associated with histone deacetylase (HDAC) activity (By similarity). Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (PubMed:18042460, PubMed:28866611). Required for maintaining open chromatin and pluripotency in embryonic stem cells (By similarity). |
| Reconstitution and Storage |
-20°C or -80°C |
| Datasheets/Manuals |
Printable datasheet for CHD1 Antibody (OACA02431) (OACA02431) |
| Application Info |
IHC: 1:20~200 |
| Immunogen |
Recombinant Human Chromodomain-helicase-DNA-binding protein 1 protein (1501-1710AA) |
| Uniprot ID |
O14646 |
| Protein Name |
Chromodomain-helicase-DNA-binding protein 1 |
| Protein Accession # |
NP_001261.2 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001270.2 |
| Gene Symbol |
CHD1 |
| Predicted Species Reactivity |
Human |
| Application |
Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | HeLa
 | Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-CDH1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the RPL30 promoter. |
| | Image 2 | Human Breast Cancer
 | Immunohistochemistry of paraffin-embedded human breast cancer using OACA02431 at dilution 1: 100 |
| | Image 3 | Human Colon Cancer
 | Immunohistochemistry of paraffin-embedded human colon cancer using OACA02431 at dilution 1: 100 |
| | Image 4 | Human Placenta
 | Immunohistochemistry of paraffin-embedded human placenta tissue using OACA02431 at dilution 1: 100 |
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