THOC1 Antibody (OACA02430)

• Product Number: OACA02430
• Name: THOC1 Antibody (OACA02430)
• Protein Size (# AA): 657 amino acids
• Molecular Weight: 76 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 9984
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: THO complex 1
• Alias Symbols: HPR1;hTREX84;nuclear matrix protein p84;P84;P84N5;THO complex subunit 1;tho1.
• Product Format: Liquid
• Reference: [1] The death domain protein p84N5, but not the short isoform p84N5s, is cell cycle-regulated and shuttles between the nucleus and the cytoplasm. Gasparri F., Sola F., Locatelli G., Muzio M.FEBS Lett. 574:13-19 (2004).
  [2] Complete sequencing and characterization of 21,243 full-length human cDNAs. Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. Sugano S.Nat. Genet. 36:40-45 (2004).
  [3] The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). The MGC Project TeamGenome Res. 14:2121-2127 (2004).
• Description of Target: Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human THO complex subunit 1 protein (428-657AA)
• Formulation: Provided in PBS, pH 7.3, 50% Glycerol, 0.02% Sodium Azide
• Uniprot ID: Q96FV9
• Protein Name: THO complex subunit 1
• Protein Accession #: NP_005122.2
• Purification: Affinity Purified
• Application Data: WB:1:500-2000, IHC:1:20-1:200
• Nucleotide Accession #: NM_005131.2
• Gene Symbol: THOC1
• Predicted Species Reactivity: Human|Mouse
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Image 1: 293T
  
  293T (1.6*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-THOC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the RHOX5 promoter.
• Image 2: Human Breast Cancer
  
  Immunohistochemistry of paraffin-embedded human breast cancer using OACA02430 at dilution 1: 100
• Image 3: Human Colon Cancer
  
  Immunohistochemistry of paraffin-embedded human colon cancer using OACA02430 at dilution 1: 100
• Image 4: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis tissue using OACA02430 at dilution 1: 100
• Image 5: Multiple samples
  
  All lanes: THOC1 antibody at 3ug/ml Lane 1: Jurkats whole cell lysate Lane 2: 293T whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: Mouse kidney tissue Secondary Goat polyclonal to Rabbit IgG at 1/10000 dilution Predicted band size: 76 kDa Observed band size: 76 kDa