STAT5B Antibody (OACA02429)

• Product Number: OACA02429
• Name: STAT5B Antibody (OACA02429)
• Protein Size (# AA): 787 amino acids
• Molecular Weight: 90 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 6777
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: signal transducer and activator of transcription 5B
• Alias Symbols: GHISID2;signal transducer and activator of transcription 5B;STAT5;transcription factor STAT5B.
• Product Format: Liquid
• Reference: [1] A Novel Missense Mutation in the SH2 Domain of the STAT5B Gene Results in a transcriptionally inactive STAT5b associated with severe IGF-I deficiency, immune dysfunction, and lack of pulmonary disease. Scaglia P.A., Martinez A.S., Feigerlova E., Bezrodnik L., Gaillard M.I., Di Giovanni D., Ballerini M.G., Jasper H.G., Heinrich J.J., Fang P., Domene H.M., Rosenfeld R.G., Hwa V. J. Clin. Endocrinol. Metab. 97:E830-839 (2012).
  [2] Growth hormone insensitivity associated with a STAT5b mutation. Kofoed E.M., Hwa V., Little B., Woods K.A., Buckway C.K., Tsubaki J., Pratt K.L., Bezrodnik L., Jasper H., Tepper A., Heinrich J.J., Rosenfeld R.G.N. Engl. J. Med. 349:1139-1147 (2003).
  [3] Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells. Chaix A., Lopez S., Voisset E., Gros L., Dubreuil P., De Sepulveda P.J. Biol. Chem. 286:5956-5966 (2011).
• Description of Target: Carries out a dual function: signal transduction and activation of transcription (PubMed:29844444). Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Signal transducer and activator of transcription 5B protein (40-280AA)
• Formulation: Provided in PBS, pH 7.3, 50% Glycerol, 0.02% Sodium Azide
• Uniprot ID: P51692
• Protein Name: Signal transducer and activator of transcription 5B
• Protein Accession #: NP_036580.2
• Purification: Affinity Purified
• Application Data: WB:1:500-2000, IHC:1:20-1:200
• Nucleotide Accession #: NM_012448.3
• Gene Symbol: STAT5B
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: MCF-7
  
  MCF-7 cells (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-STAT5B or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the AGT promoter.
• Image 2: Human Breast Cancer
  
  Immunohistochemistry of paraffin-embedded human breast cancer using OACA02429 at dilution 1: 100
• Image 3: Human Placenta
  
  Immunohistochemistry of paraffin-embedded human placenta using OACA02429 at dilution 1: 100
• Image 4: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis using OACA02429 at dilution 1: 100
• Image 5: Multiple samples
  
  All lanes: STAT5B antibody at 16 ug/ml Lane 1: HepG2 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Hale whole cell lysate Secondary Goat polyclonal to rabbit at 1/10,000 dilution Predicted band size: 90 kDa Observed band size: 90 kDa