MCM3 Antibody (OACA02425)

• Product Number: OACA02425
• Name: MCM3 Antibody (OACA02425)
• Protein Size (# AA): 808 amino acids
• Molecular Weight: 91 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 4172
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: minichromosome maintenance complex component 3
• Alias Symbols: cervical cancer proto-oncogene 5;DNA polymerase alpha holoenzyme-associated protein P1;DNA replication factor MCM3;DNA replication licensing factor MCM3;HCC5;hRlf beta subunit;MCM3 minichromosome maintenance deficient 3;minichromosome maintenance deficient 3;P1.h;p102;P1-MCM3;replication licensing factor, beta subunit;RLF subunit beta;RLFB.
• Product Format: Liquid
• Reference: [1] A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses. Menning M., Kufer T.A.FEBS Lett. 587:2137-2142 (2013).
  [2] N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454 (2012).
  [3] Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features. Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.Mol. Cell. Proteomics 11:M111.015131-M111.015131 (2012).
• Description of Target: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human DNA replication licensing factor MCM3 protein (1-295AA)
• Formulation: Provided in PBS, pH 7.3, 50% Glycerol, 0.02% Sodium Azide
• Uniprot ID: P25205
• Protein Name: DNA replication licensing factor MCM3
• Protein Accession #: NP_001257401.1
• Purification: Affinity Purified
• Application Data: WB:1:200-1000, IHC:1:20-1:200
• Nucleotide Accession #: NM_001270472.1
• Gene Symbol: MCM3
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: 293T
  
  293T (1.6*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-MCM3 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the IRF-1 promoter
• Image 2: Human Colon Cancer
  
  Immunohistochemistry of paraffin-embedded human colon cancer using OACA02425 at dilution 1: 100
• Image 3: Human Liver
  
  Immunohistochemistry of paraffin-embedded human liver using OACA02425 at dilution 1: 100
• Image 4: Human Ovarian Cancer
  
  Immunohistochemistry of paraffin-embedded human ovarian cancer using OACA02425 at dilution 1: 100
• Image 5: Human Tonsil
  
  Immunohistochemistry of paraffin-embedded human tonsil using OACA02425 at dilution 1: 100
• Image 6: Multiple samples
  
  All lanes: MCM3 antibody at 4 ug/ml Lane 1: Hela whole cell lysate Lane 2: 293T whole cell lysate Lane 3: K562 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkats whole cell lysate Lane 6: U917 whole cell lysate Secondary Goat polyclonal to rabbit at 1/10,000 dilution Predicted band size: 91 kDa Observed band size: 91 kDa