HIST1H2AI Antibody (OACA02424)

• Product Number: OACA02424
• Name: HIST1H2AI Antibody (OACA02424)
• Protein Size (# AA): 130 amino acids
• Molecular Weight: 14 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 8329|8330|8332|8336|8969
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: H2A clustered histone 11|H2A clustered histone 13|H2A clustered histone 15|H2A clustered histone 16|H2A clustered histone 17
• Alias Symbols: dJ193B12.1;dJ193B12.9;H2A histone family, member C;H2A histone family, member D;H2A histone family, member I;H2A histone family, member N;H2A histone family, member P;H2A.1;H2A.1b;H2A.i;H2A/c;H2A/d;H2A/i;H2A/n;H2A/p;H2AC11;H2AC13;H2AC14;H2AC15;H2AC16;H2AC17;H2AFC;H2AFD;H2AFI;H2AFN;H2AFP;H2AG;HIST1H2AG;HIST1H2AI;HIST1H2AK;HIST1H2AL;HIST1H2AM;histone 1, H2ag;histone 1, H2ai;histone 1, H2ak;histone 1, H2al;histone 1, H2am;histone cluster 1 H2A family member g;histone cluster 1 H2A family member i;histone cluster 1 H2A family member k;histone cluster 1 H2A family member l;histone cluster 1 H2A family member m;histone cluster 1, H2ag;histone cluster 1, H2ai;histone cluster 1, H2ak;histone cluster 1, H2al;histone cluster 1, H2am;histone H2A type 1;histone H2A type 1-J;histone H2A/p;histone H2A/ptl;pH2A/f.
• Product Format: Liquid
• Reference: [1] The human and mouse replication-dependent histone genes. Marzluff W.F., Gongidi P., Woods K.R., Jin J., Maltais L.J.Genomics 80:487-498 (2002).
  [2] The DNA sequence and analysis of human chromosome 6. Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. Beck S. Nature 425:805-811 (2003).
• Description of Target: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Histone H2A type 1 protein (2-130AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: P0C0S8
• Protein Name: Histone H2A type 1
• Protein Accession #: NP_003500.1
• Purification: Protein G Purified
• Nucleotide Accession #: NM_003509.2
• Gene Symbol: H2AC11|H2AC13|H2AC15|H2AC16|H2AC17
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.2*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the PGK1 promoter.
• Image 2: Human Lung Cancer
  
  Immunohistochemistry of paraffin-embedded human lung cancer using OACA02424 at dilution 1: 100
• Image 3: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis using OACA02424 at dilution 1: 100
• Image 4: Multiple samples
  
  All lanes: HIST1H2AG Antibody at 4 ug/ml + HL-60 whole cell lysates Secondary Goat polyclonal to rabbit at 1/10,000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa