HDAC1 Antibody (OACA02422)

• Product Number: OACA02422
• Name: HDAC1 Antibody (OACA02422)
• Protein Size (# AA): 482 amino acids
• Molecular Weight: 55 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 3065
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: histone deacetylase 1
• Alias Symbols: GON-10;HD1;histone deacetylase 1;KDAC1;reduced potassium dependency, yeast homolog-like 1;RPD3;RPD3L1.
• Product Format: Liquid
• Reference: [1] MEF-2 function is modified by a novel co-repressor, MITR. Sparrow D.B., Miska E.A., Langley E., Reynaud-Deonauth S., Kotecha S., Towers N., Spohr G., Kouzarides T., Mohun T.J.EMBO J. 18:5085-5098 (1999).
  [2] BCoR, a novel corepressor involved in BCL-6 repression. Huynh K.D., Fischle W., Verdin E., Bardwell V.J.Genes Dev. 14:1810-1823 (2000).
• Description of Target: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human Histone deacetylase 1 protein (1-482AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: Q13547
• Protein Name: Histone deacetylase 1
• Protein Accession #: NP_004955.2
• Purification: Protein G Purified
• Nucleotide Accession #: NM_004964.2
• Gene Symbol: HDAC1
• Predicted Species Reactivity: Human
• Application: Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Image 1: MCF-7
  
  MCF7 (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-HDAC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the P21 promoter.
• Image 2: Human Colon Cancer
  
  Immunohistochemistry of paraffin-embedded human colon cancer using OACA02422 at dilution 1: 100
• Image 3: Human Testis
  
  Immunohistochemistry of paraffin-embedded human testis using OACA02422 at dilution 1: 100
• Image 4: Multiple samples
  
  All lanes: HDAC1 antibody at 4ug/ml Lane 1: Mouse gonad tissue Lane 2: 293T whole cell lysates Lane 3: Hela whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: HT29 whole cell lysates Lane 6: A549 whole cell lysates Secondary Goat polyclonal to rabbit at 1/10,000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa