APEX1 Antibody (OACA02420)

Data Sheet
 
Product Number OACA02420
Product Page www.avivasysbio.com/apex1-antibody-oaca02420.html
Name APEX1 Antibody (OACA02420)
Protein Size (# AA) 318 amino acids
Molecular Weight 36 kDa
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 328
Host Rabbit
Clonality Polyclonal
Gene Full Name apurinic/apyrimidinic endodeoxyribonuclease 1
Alias Symbols AP endonuclease class I;AP lyase;APE;APE1;APEN;APEX;APEX nuclease;APEX nuclease (multifunctional DNA repair enzyme) 1;apurinic/apyrimidinic (abasic) endonuclease;apurinic-apyrimidinic endonuclease 1;APX;deoxyribonuclease (apurinic or apyrimidinic);DNA-(apurinic or apyrimidinic site) endonuclease;DNA-(apurinic or apyrimidinic site) lyase;HAP1;protein REF-1;redox factor-1;REF1;REF-1.
Product Format Liquid
Description of Target Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
Reconstitution and Storage -20°C or -80°C
Datasheets/Manuals Printable datasheet for APEX1 Antibody (OACA02420) (OACA02420)
Specificity Human
Immunogen Recombinant Human DNA-(apurinic or apyrimidinic site) lyase protein (32-318AA)
Formulation Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
Uniprot ID P27695
Protein Name DNA-(apurinic or apyrimidinic site) lyase
Protein Accession # NP_001231178.1
Purification Protein G Purified
Nucleotide Accession # NM_001244249.1
Gene Symbol APEX1
Predicted Species Reactivity Human|Mouse
Application Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
Predicted Homology Based on Immunogen Sequence Human, Mouse
Image 1
MCF-7
MCF7 (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-APE1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the MDR1 promoter.
Image 2
Human Lung
Immunohistochemistry of paraffin-embedded human lungs using OACA02420 at dilution 1: 100
Image 3
Human Testis
Immunohistochemistry of paraffin-embedded human testis using OACA02420 at dilution 1: 100
Image 4
Human Tonsil
Immunohistochemistry of paraffin-embedded human tonsil using OACA02420 at dilution 1: 100
Image 5
Multiple samples
Western blot All lanes: DNA-(apurinic or apyrimidinic site) lyase antibody at 2 ug/ml Lane 1: Hela whole cell lysate Lane 2: Mouse brain tissue Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raji whole cell lysate Lane 6: A549 whole cell lysate Lane 7: K562 whole cell lysate Lane 8: Mouse liver tissue Lane 9: NIH3T3 whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 36 kDa Observed band size: 36; 34; 21 kDa
 

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