AKT1 Antibody (OACA02419)

• Product Number: OACA02419
• Name: AKT1 Antibody (OACA02419)
• Protein Size (# AA): 480 amino acids
• Molecular Weight: 56 kDa
• Isotype: IgG
• Conjugation: Unconjugated
• NCBI Gene Id: 207
• Host: Rabbit
• Clonality: Polyclonal
• Gene Full Name: AKT serine/threonine kinase 1
• Alias Symbols: AKT;AKT1m;PKB;PKB alpha;PKB-ALPHA;PRKBA;Protein kinase B;protein kinase B alpha;proto-oncogene c-Akt;RAC;rac protein kinase alpha;RAC-ALPHA;RAC-alpha serine/threonine-protein kinase;RAC-PK-alpha;serine-threonine protein kinase;v-akt murine thymoma viral oncogene homolog 1;v-akt murine thymoma viral oncogene-like protein 1.
• Product Format: Liquid
• Reference: [1] Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily. Jones P.F., Jakubowicz T., Pitossi F.J., Maurer F., Hemmings B.A.Proc. Natl. Acad. Sci. U.S.A. 88:4171-4175 (1991)
• Description of Target: AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI(3)P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.
• Reconstitution and Storage: -20°C or -80°C
• Specificity: Human
• Immunogen: Recombinant Human RAC-alpha serine/threonine-protein kinase protein (1-480AA)
• Formulation: Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
• Uniprot ID: P31749
• Protein Name: RAC-alpha serine/threonine-protein kinase
• Protein Accession #: NP_001014431.1
• Purification: Protein G Purified
• Nucleotide Accession #: NM_001014431.1
• Gene Symbol: AKT1
• Predicted Species Reactivity: Human
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunoprecipitation|Western blot
• Predicted Homology Based on Immunogen Sequence: Human
• Image 1: HeLa
  
  Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-AKT1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the exon-1 of Egr1 promoter
• Image 2: Human Cervical Cancer
  
  Immunohistochemistry of paraffin-embedded human cervical cancer using OACA02419 at dilution 1: 100
• Image 3: Human Prostate
  
  Immunohistochemistry of paraffin-embedded human prostate tissue using OACA02419 at dilution 1: 100
• Image 4: Human Tonsil
  
  Immunohistochemistry of paraffin-embedded human tonsil tissue using OACA02419 at dilution 1: 100
• Image 5: Multiple samples
  
  All lanes: RAC-alpha serine/threonine-protein kinase antibody at 2 ug/ml Lane 1: Colo320 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15,000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa