AGO2 Antibody (OACA02418)

Data Sheet
 
Product Number OACA02418
Product Page www.avivasysbio.com/ago2-antibody-oaca02418.html
Name AGO2 Antibody (OACA02418)
Protein Size (# AA) 859 amino acids
Molecular Weight 97 kDa
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 27161
Host Rabbit
Clonality Polyclonal
Gene Full Name argonaute RISC catalytic component 2
Alias Symbols argonaute 2, RISC catalytic component;Argonaute RISC catalytic component 2;cancer susceptibility candidate 7 (non-protein coding);CASC7;EIF2C2;Eukaryotic translation initiation factor 2C 2;eukaryotic translation initiation factor 2C, 2;LESKRES;LINC00980;long intergenic non-protein coding RNA 980;PAZ Piwi domain protein;PPD;protein argonaute-2;protein slicer;Q10.
Product Format Liquid
Reference [1] The crystal structure of human Argonaute2. Schirle N.T., MacRae I.J. Science 336:1037-1040 (2012).
[2] The structure of human argonaute-2 in complex with miR-20a. Elkayam E., Kuhn C.D., Tocilj A., Haase A.D., Greene E.M., Hannon G.J., Joshua-Tor L. Cell 150:100-110 (2012).
[3] Structural analysis of 5\'-mRNA-cap interactions with the human AGO2 MID domain. Frank F., Fabian M.R., Stepinski J., Jemielity J., Darzynkiewicz E., Sonenberg N., Nagar B. EMBO Rep. 12:415-420 (2011).
Description of Target Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
Reconstitution and Storage -20°C or -80°C
Datasheets/Manuals Printable datasheet for AGO2 Antibody (OACA02418) (OACA02418)
Specificity Human
Immunogen Recombinant Human Protein argonaute-2 protein (517-818AA)
Formulation Provided in PBS, pH 7.4, 50% Glycerol, 0.03% Proclin 300
Uniprot ID Q9UKV8
Protein Name Protein argonaute-2
Protein Accession # NP_001158095.1
Purification Protein G Purified
Nucleotide Accession # NM_001164623.1
Gene Symbol AGO2
Predicted Species Reactivity Human|Mouse
Application Chromatin immunoprecipitation|Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot
Predicted Homology Based on Immunogen Sequence Human, Mouse
Image 1
HeLa
Hela (1.1*10e6) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4 ug anti-AgO2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the SLC1A5 promoter.
Image 2
Human Breast Cancer
Immunohistochemistry of paraffin-embedded human breast cancer using OACA02418 at dilution 1: 100
Image 3
Human Breast Cancer
Immunohistochemistry of paraffin-embedded human breast cancer using OACA02418 at dilution 1: 100
Image 4
Human Gastric Cancer
Immunohistochemistry of paraffin-embedded human gastric cancer using OACA02418 at dilution 1: 100
Image 5
Multiple samples
All lanes: Protein argonaute-2 antibody at 2 ug/ml Lane 1: A549 whole cell lysate Lane 2: Jurkats whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Raw264.7 whole cell lysate Lane 6: K562 whole cell lysate Lane 7: Mouse liver tissue Lane 8: Mouse kidney tissue Goat polyclonal to Rabbit IgG at 1/10,000 dilution Predicted band size: 97 kDa Observed band size: 90 kDa
 

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