| Product Number |
OACA00078 |
| Product Page |
www.avivasysbio.com/arhgdia-antibody-oaca00078.html |
| Name |
ARHGDIA Antibody (OACA00078) |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
396 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Purity |
> 95% by Caprylic Acid Ammonium Sulfate Precipitation |
| Gene Full Name |
Rho GDP dissociation inhibitor alpha |
| Alias Symbols |
epididymis secretory sperm binding protein Li 47e;GDIA1;GDP-dissociation inhibitor, aplysia RAS-related 1;HEL-S-47e;NPHS8;Rho GDP dissociation inhibitor (GDI) alpha;rho GDP-dissociation inhibitor 1;RHOGDI;Rho-GDI alpha;RHOGDI-1. |
| Product Format |
Liquid |
| Reference |
[1] Identification of two human Rho GDP dissociation inhibitor proteins whose overexpression leads to disruption of the actin cytoskeleton.'Leffers H., Nielsen M.S., Andersen A.H., Honore B., Madsen P., Vandekerckhove J., Celis J.E.Exp. Cell Res. 209:165-174(1993)
[2] Molecular cloning of human rho GDI.'Maeda A., Kaibuchi K., Takai Y.
[3] cDNA sequence of human rho GDP dissociation inhibitor.'Chuang T.H., Bokoch G.M.
[4] Genomic sequence of a human rho GDP dissociation inhibitor (GDI).'Mulheron J.G., Schwinn D.A., Caron M.G., Liggett S.B. |
| Description of Target |
Controls Rho proteins homeostasis. Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Retains Rho proteins such as CDC42, RAC1 and RHOA in an inactive cytosolic pool, regulating their stability and protecting them from degradation. Actively involved in the recycling and distribution of activated Rho GTPases in the cell, mediates extraction from membranes of both inactive and activated molecules due its exceptionally high affinity for prenylated forms. Through the modulation of Rho proteins, may play a role in cell motility regulation. In glioma cells, inhibits cell migration and invasion by mediating the signals of SEMA5A and PLXNB3 that lead to inactivation of RAC1. |
| Reconstitution and Storage |
-20°C or -80°C |
| Datasheets/Manuals |
Printable datasheet for ARHGDIA Antibody (OACA00078) (OACA00078) |
| Application Info |
ELISA: Use at an assay dependent dilution
WB: 1:500 ~ 2000
IHC: 1:20 ~ 200 |
| Immunogen |
Recombinant Human Rho GDP-dissociation inhibitor 1 protein (2-204AA) |
| Uniprot ID |
P52565 |
| Protein Name |
Rho GDP-dissociation inhibitor 1 |
| Protein Accession # |
NP_001172006.1; NP_001172007.1; NP_001288169.1; NP_001288170.1; NP_001288171.1; NP_001288172.1; NP_004300.1 |
| Purification |
Protein G Purified |
| Nucleotide Accession # |
NM_001185077.2; NM_001185078.2; NM_001301240.1; NM_001301241.1; NM_001301242.1; NM_001301243.1; NM_004309.5 |
| Gene Symbol |
ARHGDIA |
| Predicted Species Reactivity |
Human |
| Application |
Enzyme-linked immunosorbent assay|Immunohistochemistry|Western blot |
| Image 1 | EC109 , 293T
 | All lanes : Rho GDP-dissociation inhibitor 1 antibody at2 µg/ml
Lane 1 :EC109 whole cell lysate
Lane 2 :293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 22.4 kDa
Observed band size: 40 kDa
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