ULK1 Antibody (Phospho-Ser556) (OABF01248)

Data Sheet
 
Product Number OABF01248
Product Page www.avivasysbio.com/ulk1-antibody-phospho-ser556-oabf01248.html
Name ULK1 Antibody (Phospho-Ser556) (OABF01248)
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 8408
Host Rabbit
Clonality Polyclonal
Concentration 1 ug/ul
Gene Full Name unc-51 like autophagy activating kinase 1
Description
Alias Symbols ATG1;ATG1 autophagy related 1 homolog;ATG1A;autophagy-related protein 1 homolog;hATG1;serine/threonine-protein kinase ULK1;UNC51;Unc51.1;Unc-51-like kinase 1.
Product Format Liquid. Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Description of Target Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy (PubMed:25040165).
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for ULK1 Antibody (Phospho-Ser556) (OABF01248)
Additional Information Subcellular location: Cytoplasm
Application Info WB: 1:100~1000
IHC-P: 1:100~500
Fluorescent-IHC: 1:50~200
Immunogen KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556
Warning This product contains sodium azide, which forms hydrazoic acid under acidic conditions and may react with lead or copper plumbing to produce explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
Intended Use For research use only. Not intended for diagnostic or therapeutic use.
Uniprot ID O75385
Protein Name Serine/threonine-protein kinase ULK1
Publications

Mir214-3p and Hnf4a/Hnf4α reciprocally regulate Ulk1 expression and autophagy in nonalcoholic hepatic steatosis. Autophagy. NULL, 1-17 (2020). 33078654

The Combination of Fasting, Acute Resistance Exercise, and Protein Ingestion Led to Different Responses of Autophagy Markers in Gastrocnemius and Liver Samples. Nutrients. 12, (2020). 32121154

Purification Protein A Purified
Gene Symbol ULK1
Predicted Species Reactivity Horse|Human|Mouse|Porcine|Rat
Application Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
Image 1
Rat brain
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-ATG1 (Ser556) Polyclonal Antibody, Unconjugated (OABF01248) at 1:200 followed by conjugation to the secondary antibody and DAB staining
 

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