EIF2AK4 Antibody (Phospho-Thr667) (OABF01173)

Data Sheet
 
Product Number OABF01173
Product Page www.avivasysbio.com/eif2ak4-antibody-phospho-thr667-oabf01173.html
Name EIF2AK4 Antibody (Phospho-Thr667) (OABF01173)
Isotype IgG
Conjugation Unconjugated
NCBI Gene Id 440275
Host Rabbit
Clonality Polyclonal
Concentration 1 ug/ul
Gene Full Name eukaryotic translation initiation factor 2 alpha kinase 4
Description
Alias Symbols eIF-2-alpha kinase GCN2;Eukaryotic translation initiation factor 2-alpha kinase 4;GCN2;GCN2 eIF2alpha kinase;GCN2-like protein;general control nonderepressible 2;PVOD2.
Product Format Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
Description of Target Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).
Reconstitution and Storage -20°C
Datasheets/Manuals Printable datasheet for EIF2AK4 Antibody (Phospho-Thr667) (OABF01173)
Specificity This phosphorylation site is homologous to Thr666 in Mouse and Thr667 in Rat
Additional Information Modification: Phosphorylation (Thr667)
Application Info WB: 1:300-1000
IHC-P: 1:200-400
Fluorescent-IHC: 1:50-200
Optimal dilutions should be determined by the end user.
Immunogen KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667
Uniprot ID Q9P2K8
Protein Name eIF-2-alpha kinase GCN2
Publications

Effects of the presence and absence of amino acids on translation, signaling, and long-term depression in hippocampal slices from Fmr1 knockout mice. J Neurochem. 151, 764-776 (2019). 31539452

Purification Protein A Purified
Gene Symbol EIF2AK4
Predicted Species Reactivity Bovine|Dog|Horse|Human|Mouse|Rat
Application Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
Image 1
Mouse liver
Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti phospho-GCN2 (Thr667) Polyclonal Antibody, Unconjugated (OABF01173) at 1:200 followed by conjugation to the secondary antibody and DAB staining
 

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