| Product Number |
OABF00275 |
| Product Page |
www.avivasysbio.com/hif1a-antibody-oabf00275.html |
| Name |
HIF1A Antibody (OABF00275) |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
3091 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
1 ug/ul |
| Gene Full Name |
hypoxia inducible factor 1 subunit alpha |
| Alias Symbols |
ARNT interacting protein;ARNT-interacting protein;basic-helix-loop-helix-PAS protein MOP1;bHLHe78;class E basic helix-loop-helix protein 78;HIF1;HIF-1A;HIF-1alpha;HIF-1-alpha;HIF1-ALPHA;hypoxia inducible factor 1 alpha subunit;hypoxia inducible factor 1, |
| Product Format |
Liquid. Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. |
| Description of Target |
Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Activation requires recruitment of transcriptional coactivators such as CREBBP and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia. |
| Reconstitution and Storage |
-20°C |
| Datasheets/Manuals |
Printable datasheet for HIF1A Antibody (OABF00275) |
| Additional Information |
Subcellular location: Cytoplasm, Nucleus |
| Application Info |
WB: 1:100 - 1000
IHC-P: : 1:100 - 500 |
| Immunogen |
KLH conjugated synthetic peptide derived from human of human HIF-1 Alpha |
| Uniprot ID |
Q16665 |
| Protein Name |
Hypoxia-inducible factor 1-alpha |
| Purification |
Protein A Purified |
| Gene Symbol |
HIF1A |
| Predicted Species Reactivity |
Bovine|Dog|Human|Mouse|Other|Porcine|Rabbit|Rat |
| Application |
Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| Image 1 | KLN 205 cells
 | KLN 205 cells labeled with Rabbit HIF-1 Alpha Polyclonal Antibody Polyclonal Antibody, Unconjugated (OABF00275) followed by a FITC Conjugated Secondary Antibody. |
| | Image 2 | L1 mouse heart lysates, L2 mouse large intestine lysates
 | L1 mouse heart lysates, L2 mouse large intestine lysates probed with Anti HIF-1 Alpha Polyclonal Antibody, Unconjugated (OABF00275) at 1:200 in 4C. Followed by conjugation to secondary antibody at 1:3000 90min in 37C. Predicted band and observed band size: 92kD |
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