BAG2 Antibody (OABB02007)

• Product Number: OABB02007
• Name: BAG2 Antibody (OABB02007)
• Molecular Weight: 23772 MW
• Clone: Polyclonal
• Isotype: Rabbit IgG
• NCBI Gene Id: 9532
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 500 ug/ml
• Gene Full Name: BAG cochaperone 2
• Description: Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 2(BAG2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
• Alias Symbols: BAG family molecular chaperone regulator 2;BAG-2;BCL2 associated athanogene 2;bcl-2-associated athanogene 2;dJ417I1.2;dJ417I1.2 (BAG-family molecular chaperone regulator 2).
• Peptide Sequence: Synthetic peptide located within the following region: MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYSACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRFN
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Reference: 1. Takayama S, Xie Z, Reed JC (Feb 1999). "An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators". J Biol Chem 274 (2): 781–6.
  2. "Entrez Gene: BAG2 BCL2-associated athanogene 2".
  
• Description of Target: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release (PubMed:24318877, PubMed:9873016).
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: No cross reactivity with other proteins.
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
• Additional Information: Background: BAG family molecular chaperone regulator 2 is a protein that in humans is encoded by the BAG2 gene. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. The functional antagonisms displayed between BAG family proteins and Hip suggest that a proper balance of these 2 types of protein is required for achieving optimal cycles of substrate binding and release required for inducting conformational changes in proteins, with Hip promoting peptide substrate binding by Hsc70/Hsp70 and BAG family proteins promoting dissociation.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Mouse, Rat: By Heat
• Immunogen: E.coli-derived human BAG2 recombinant protein (Position: M1-N211). Human BAG2 shares 93.4% amino acid (aa) sequence identity with mouse BAG2.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Uniprot ID: O95816
• Protein Name: BAG family molecular chaperone regulator 2
• Purification: Affinity Purified
• Tested Species Reactivity: Human, Mouse, Rat
• Gene Symbol: BAG2
• Predicted Species Reactivity: Human
• Application: Flow cytometry|Immunocytochemistry|Immunofluorescence|Western blot
• Image 1: Human Pancreatic Cancer Tissue
  
  Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
  IHC(P): Human Pancreatic Cancer Tissue
• Image 2: Mouse Testis Tissue
  
  Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
  IHC(P): Mouse Testis Tissue
• Image 3: Rat Testis Tissue
  
  Anti-BAG2 Picoband antibody, OABB02007, IHC(P)
  IHC(P): Rat Testis Tissue
• Image 4: Rat Testis Tissue Lysate, HELA Whole Cell Lysate, A549 Whole Cell Lysate
  
  Anti-BAG2 Picoband antibody, OABB02007, Western blotting
  All lanes: Anti BAG2 (OABB02007) at 0.5ug/ml
  Lane 1: Rat Testis Tissue Lysate at 50ug
  Lane 2: HELA Whole Cell Lysate at 40ug
  Lane 3: A549 Whole Cell Lysate at 40ug
  Predicted bind size: 24KD
  Observed bind size: 24KD