CD44 Antibody - N-terminal region (OABB01816)

• Product Number: OABB01816
• Name: CD44 Antibody - N-terminal region (OABB01816)
• Molecular Weight: 81538 MW
• Clone: Polyclonal
• Isotype: Rabbit IgG
• NCBI Gene Id: 960
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 500 ug/ml
• Gene Full Name: CD44 molecule (Indian blood group)
• Description: Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P, IF in Human;Rat.
• Alias Symbols: CD44 antigen;CDW44;cell surface glycoprotein CD44;chondroitin sulfate proteoglycan 8;CSPG8;ECMR-III;epican;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;HCELL;hematopoietic cell E- and L-selectin ligand;heparan sulfate proteoglycan;Hermes antigen;homing function and Indian blood group system;HUTCH-I;hyaluronate receptor;IN;Indian blood group antigen;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;phagocytic glycoprotein 1;Phagocytic glycoprotein I;soluble CD44.
• Peptide Sequence: Synthetic peptide located within the following region: RFAGVFHVEKNGRYSISRTEAADLCKAF
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reference: 1. Fagan-Solis KD, et al. Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer. Mol Cancer, 2014 Jul 2.
  2. Zhang P, et al. CD44 variant, but not standard CD44 isoforms, mediate disassembly of endothelial VE-cadherin junction on metastatic melanoma cells. FEBS Lett, 2014 Dec 20.
  
• Description of Target: Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function (By similarity).
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: No cross reactivity with other proteins.
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
• Additional Information: Background: CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
  Immunofluorescence: 2 ug/ml: Human, Rat
• Immunogen: Polypeptide
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Uniprot ID: P16070
• Protein Name: CD44 antigen
• Purification: Affinity Purified
• Tested Species Reactivity: Human, Rat
• Gene Symbol: CD44
• Predicted Species Reactivity: Human|Rat
• Application: Immunofluorescence|Immunohistochemistry|Western blot
• Image 1: Human Intestinal Cancer Tissue
  
  Anti-CD44 Picoband antibody, OABB01816, IHC(P)
  IHC(P): Human Intestinal Cancer Tissue
• Image 2: HELA Whole Cell Lysate, PANC Whole Cell Lysate
  
  Anti-CD44 Picoband antibody, OABB01816, Western blotting
  All lanes: Anti CD44 (OABB01816) at 0.5ug/ml
  Lane 1: HELA Whole Cell Lysate at 40ug
  Lane 2: PANC Whole Cell Lysate at 40ug
  Predicted bind size: 82KD
  Observed bind size: 115KD