Product Number |
OABB01783 |
Product Page |
www.avivasysbio.com/neuropilin-1-antibody-oabb01783.html |
Name |
NEUROPILIN 1 Antibody (OABB01783) |
Molecular Weight |
103134 MW |
Clone |
Polyclonal |
Isotype |
Rabbit IgG |
NCBI Gene Id |
8829 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
500 ug/ml |
Gene Full Name |
neuropilin 1 |
Description |
Rabbit IgG polyclonal antibody for Neuropilin-1(NRP1) detection. Tested with WB, IHC-P in Human;Rat. |
Alias Symbols |
BDCA4;CD304;neuropilin-1;NP1;NRP;transmembrane receptor;vascular endothelial cell growth factor 165 receptor;VEGF165R. |
Peptide Sequence |
Synthetic peptide located within the following region: KHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFPTYGFNCEFGWGSHKTFCHWEHDNHVQLKWSVLTSKTGPIQDHTGDGNFIYSQADENQKGKVARLVSPVVYSQNSAHCMTFWYHMSGSHVGTLRVKLRYQKPEEYDQLVWMAIGHQGDHWKEGRVLLHKSLKLYQVIFEGEIGKGNLGGIAVDDISINNHISQEDCAKPADLDKKNPEIKIDET |
Product Format |
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Reference |
1. Gagnon, M. L., Bielenberg, D. R., Gechtman, Z., Miao, H.-Q., Takashima, S., Soker, S., Klagsbrun, M. Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor: in vivo expression and antitumor activity. Proc. Nat. Acad. Sci. 97: 2573-2578, 2000. 2. Gu, C., Rodriguez, E. R., Riemert, D. V., Shu, T., Fritzsch, B., Richards, L. J., Kolodkin, A. L., Ginty, D. D. Neuropilin-1 conveys semaphorin and VEGF signaling during neural and cardiovascular development. Dev. Cell 5: 45-57, 2003.
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Description of Target |
The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulate VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). |
Reconstitution and Storage |
2°C to 8°C|-20°C |
Datasheets/Manuals |
Printable datasheet for NEUROPILIN 1 Antibody (OABB01783) |
Specificity |
No cross reactivity with other proteins. |
Additional Information |
Notes: WB: The detection limit for Neuropilin 1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
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Additional Information |
Background: This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. |
Application Info |
Western blot: 0.1-0.5 ug/ml: Human, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Immunogen |
E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. |
Storage Buffer |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Uniprot ID |
O14786 |
Protein Name |
Neuropilin-1 |
Purification |
Affinity Purified |
Tested Species Reactivity |
Human, Rat |
Gene Symbol |
NRP1 |
Predicted Species Reactivity |
Human|Rat |
Application |
Immunohistochemistry|Western blot |
Image 1 | Human Placenta Tissue
| Anti-Neuropillin-1 Picoband antibody, OABB01783, IHC(P) IHC(P): Human Placenta Tissue |
| Image 2 | U87 Whole Cell Lysate, A549 Whole Cell Lysate, Human Placenta Tissue Lysate, Rat Cardiac Muscle Tissue Lysate
| Anti-Neuropillin-1 Picoband antibody, OABB01783, Western blotting All lanes: Anti Neuropillin-1 (OABB01783) at 0.5ug/ml Lane 1: U87 Whole Cell Lysate at 40ug Lane 2: A549 Whole Cell Lysate at 40ug Lane 3: Human Placenta Tissue Lysate at 50ug Lane 4: Rat Cardiac Muscle Tissue Lysate at 50ug Predicted bind size: 103KD Observed bind size: 103KD |
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