| Product Number |
OABB01538 |
| Product Page |
www.avivasysbio.com/bdnf-antibody-oabb01538.html |
| Name |
BDNF Antibody (OABB01538) |
| Molecular Weight |
28123 MW |
| Clone |
Polyclonal |
| Isotype |
Rabbit IgG |
| NCBI Gene Id |
12064 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
500 ug/ml |
| Gene Full Name |
brain derived neurotrophic factor |
| Description |
Rabbit IgG polyclonal antibody for Brain-derived neurotrophic factor(BDNF) detection. Tested with WB, IHC-P, IHC-F, ICC, FCM in Human;Mouse;Rat. |
| Alias Symbols |
brain-derived neurotrophic factor. |
| Product Format |
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Description of Target |
During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for BDNF Antibody (OABB01538) |
| Specificity |
No cross reactivity with other proteins. |
| Additional Information |
Notes: WB: The detection limit for BDNF is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
|
| Additional Information |
Background: Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system. |
| Application Info |
Western blot: 0.1-0.5 ug/ml: Mouse, Rat: Human
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: Human: By Heat
Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Human
Immunocytochemistry: 0.5-1 ug/ml: Human
Flow Cytometry: 1-3 ug/1x10^6 cells: Human |
| Immunogen |
Anti-BDNF Picoband Antibody (PB9075) was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF. |
| Storage Buffer |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Uniprot ID |
P21237 |
| Protein Name |
Brain-derived neurotrophic factor |
| Purification |
Affinity Purified |
| Tested Species Reactivity |
Mouse, Rat |
| Gene Symbol |
Bdnf |
| Predicted Species Reactivity |
Bovine|Dog|Horse|Human|Monkey|Mouse|Rabbit|Rat |
| Application |
Flow cytometry|Immunocytochemistry|Immunohistochemistry|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | Mouse Brain Tissue
 | Anti-BDNF antibody
IHC(P): Mouse Brain Tissue |
|
| Image 2 | Rat Brain Tissue
 | Anti-BDNF antibody
IHC(P): Rat Brain Tissue |
|
| Image 3 | Rat Brain Tissue Lysate
 | Anti-BDNF antibody
All lanes: Anti-BDNF(OABB01538) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 28 kDa
Observed bind size: 28 kDa
|
|
