OLR1 Polyclonal Antibody (OABB00784)

• Product Number: OABB00784
• Name: OLR1 Polyclonal Antibody (OABB00784)
• Molecular Weight: 30959 MW
• Clone: Polyclonal
• Isotype: Rabbit IgG
• NCBI Gene Id: 4973
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 500 ug/ml
• Gene Full Name: oxidized low density lipoprotein receptor 1
• Description: Rabbit IgG polyclonal antibody for Oxidized low-absorbance lipoprotein receptor 1(OLR1) detection. Tested with WB in Human;Rat.
• Alias Symbols: CLEC8A;C-type lectin domain family 8 member A;hLOX-1;Lectin-like oxidized LDL receptor 1;lectin-type oxidized LDL receptor 1;LOX1;LOXIN;ox LDL receptor 1;oxidized low density lipoprotein (lectin-like) receptor 1;oxidized low-density lipoprotein receptor 1;oxidized low-density lipoprotein receptor 1, soluble form;SCARE1;scavenger receptor class E, member 1;SLOX1.
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reference: 1. Aoyama, T., Sawamura, T., Furutani, Y., Matsuoka, R., Yoshida, M. C., Fujiwara, H., Masaki,
  T. Structure and chromosomal assignment of the human lectin-like oxidized low-density-lipoprotein
  receptor-1 (LOX-1) gene. Biochem. J. 339: 177-184, 1999.
  2. Delneste, Y., Magistrelli, G., Gauchat, J.-F., Haeuw, J.-F., Aubry, J.-P., Nakamura, K.,
  Kawakami-Honda, N., Goetsch, L., Sawamura, T., Bonnefoy, J.-Y., Jeannin, P. Involvement of LOX-1
  in dendritic cell-mediated antigen cross-presentation. Immunity 17: 353-362, 2002.
  3. Luedecking-Zimmer, E., DeKosky, S. T., Chen, Q., Barmada, M. M., Kamboh, M. I. Investigation of
  oxidized LDL-receptor 1 (OLR1) as the candidate gene for Alzheimer's disease on chromosome
  12. Hum. Genet. 111: 443-451, 2002
• Description of Target: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: No cross reactivity with other proteins.
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
• Additional Information: Background: OLR1(oxidized low absorbance lipoprotein(lectin-like) receptor 1) also called CLEC8A, LOX-1, SCARE1, is a receptor protein which belongs to the C-type lectin superfamily. The OLR1 gene encodes a cell-surface endocytosis receptor for oxidized low absorbance lipoprotein(OxLDL). The OLR1 gene is mapped on 12p13.2. Incubation of the cells with LDL had no effect on LOX1 expression, but incubation with OxLDL resulted in a dose-dependent increase in LOX1 mRNA and protein expression; however, very high concentrations of OxLDL caused a decrease in OxLDL expression, perhaps indicating toxic effects on endothelial cells. LOX1 was also expressed in macrophages, but not in vascular smooth muscle cells. The findings suggested a role for LOX1 in the pathophysiology of atherosclerotic cardiovascular disease. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
• Immunogen: Polypeptide
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Uniprot ID: P78380
• Protein Name: Oxidized low-density lipoprotein receptor 1
• Publications:

  Membrane Cholesterol Modulates LOX-1 Shedding in Endothelial Cells. PLoS ONE. 10, e0141270 (2015). 26495844

• Purification: Affinity Purified
• Tested Species Reactivity: Human, Rat
• Gene Symbol: OLR1
• Predicted Species Reactivity: Human
• Application: Western blot
• Image 1: Rat Liver tissue and HeLa, K562, Raji cells
  
  Anti- LOX1 antibody, OABB00784, Western blotting All lanes: Anti LOX1 (OABB00784) at 0.5ug/ml Lane 1: Rat Liver Tissue Lysate at 50ug
  Lane 2: HELA Whole Cell Lysate at 40ug
  Lane 3: K562 Whole Cell Lysate at 40ug
  Lane 4: HELA Whole Cell Lysate at 40ug
  Lane 5: RAJI Whole Cell Lysate at 40ug
  Predicted bind size: 31KD
  Observed bind size: 38KD