| Product Number |
OABB00620 |
| Product Page |
www.avivasysbio.com/nox2-polyclonal-antibody-oabb00620.html |
| Name |
NOX2 Polyclonal Antibody (OABB00620) |
| Molecular Weight |
65336 MW |
| Clone |
Polyclonal |
| Isotype |
Rabbit IgG |
| NCBI Gene Id |
1536 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
500 ug/ml |
| Gene Full Name |
cytochrome b-245 beta chain |
| Description |
|
| Alias Symbols |
AMCBX2;CGD;CGD91-phox;CGDX;cytochrome b(558) subunit beta;cytochrome b-245 beta polypeptide;cytochrome b-245 heavy chain;cytochrome b558 subunit beta;GP91-1;GP91PHOX;GP91-PHOX;heme-binding membrane glycoprotein gp91phox;IMD34;NADPH oxidase 2;neutrophil cytochrome b 91 kDa polypeptide;NOX2;p22 phagocyte B-cytochrome;p91-PHOX;superoxide-generating NADPH oxidase heavy chain subunit. |
| Product Format |
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Reference |
1. Bionda, C., Li, X. J., van Bruggen, R., Eppink, M., Roos, D., Morel, F., Stasia, M.-J. Functional
analysis of two-amino acid substitutions in gp91phox in a patient with X-linked flavocytochrome
b(558)-positive chronic granulomatous disease by means of transgenic PLB-985 cells. Hum. Genet.
115: 418-427, 2004.
2. Bolscher, B. G. J. M., de Boer, M., de Klein, A., Weening, R. S., Roos, D. Point mutations in the
beta-subunit of cytochrome b(558) leading to X-linked chronic granulomatous disease. Blood 77:
2482-2487, 1991.
3. "Entrez Gene: CYBB cytochrome b-245, beta polypeptide (chronic granulomatous disease)" |
| Description of Target |
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for NOX2 Polyclonal Antibody (OABB00620) |
| Specificity |
No cross reactivity with other proteins. |
| Additional Information |
Notes: Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
|
| Additional Information |
Background: NOX2(NADPH OXIDASE 2), also called CYBB(CYTOCHROME b(-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species(ROS) in a process dependent on microtubules(X-ROS signaling). |
| Application Info |
Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat |
| Immunogen |
Polypeptide |
| Storage Buffer |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Uniprot ID |
P04839 |
| Protein Name |
Cytochrome b-245 heavy chain |
| Publications |
Different signalling in infarcted and non-infarcted areas of rat failing hearts: A role of necroptosis and inflammation. J Cell Mol Med. 23, 6429-6441 (2019). 31328381 |
| Purification |
Affinity Purified |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
CYBB |
| Predicted Species Reactivity |
Hamster|Human|Mouse|Rat |
| Application |
Immunohistochemistry|Western blot |
| Predicted Homology Based on Immunogen Sequence |
Human; Mouse; Rat |
| Image 1 | Human Intestinal Cancer
 | IHC(P):Human Intestinal Cancer Tissue |
|
| Image 2 | Hela, Jurkat, MCF-7, SMMC
 | Lane1: Hela Cell Lysate
Lane2: Jurkat Cell Lysate
Lane3: MCF-7 Cell Lysate
Lane4: SMMC Cell Lysate |
|
