| Product Number |
OABB00446 |
| Product Page |
www.avivasysbio.com/gclc-antibody-oabb00446.html |
| Name |
GCLC Antibody (OABB00446) |
| Molecular Weight |
72766 MW |
| Clone |
Polyclonal |
| Isotype |
Rabbit IgG |
| NCBI Gene Id |
2729 |
| Clonality |
Polyclonal |
| Concentration |
500 ug/ml |
| Gene Full Name |
glutamate-cysteine ligase catalytic subunit |
| Description |
Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human;Mouse;Rat. |
| Alias Symbols |
gamma-ECS;gamma-glutamylcysteine synthetase;GCL;GCS;GCS heavy chain;GLCL;GLCLC;glutamate--cysteine ligase catalytic subunit. |
| Product Format |
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Reference |
1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for
human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35,
1992.
2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M.
L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine
synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995. |
| Description of Target |
Glutamate--cysteine ligase catalytic subunit |
| Reconstitution and Storage |
2°C to 8°C|-20°C |
| Datasheets/Manuals |
Printable datasheet for GCLC Antibody (OABB00446) |
| Specificity |
No cross reactivity with other proteins. |
| Additional Information |
Notes: Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
|
| Additional Information |
Background: GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. |
| Application Info |
Western blot: 0.1-0.5 ug/ml: Rat: Human, Mouse
Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat |
| Immunogen |
Polypeptide |
| Storage Buffer |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Uniprot ID |
P48506 |
| Protein Name |
Glutamate--cysteine ligase catalytic subunit |
| Protein Accession # |
NP_001184044.1; NP_001489.1 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001197115.1; NM_001498.3 |
| Tested Species Reactivity |
Human, Rat |
| Gene Symbol |
GCLC |
| Predicted Species Reactivity |
Bovine|Human|Mouse|Rat |
| Application |
Immunohistochemistry|Western blot |
| Image 1 | Rat Brain,Kidney,Heart, Lung
 | Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Heart Tissue Lysate
Lane 1: Rat Lung Tissue Lysate |
|
