MBP Polyclonal Antibody (OABB00147)

• Product Number: OABB00147
• Name: MBP Polyclonal Antibody (OABB00147)
• Molecular Weight: 33117 MW
• Clone: Polyclonal
• Isotype: Rabbit IgG
• NCBI Gene Id: 4155
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 500 ug/ml
• Gene Full Name: myelin basic protein
• Description:
  View product citations for MBP Polyclonal Antibody (OABB00147) on CiteAb
• Alias Symbols: Golli-MBP;myelin A1 protein;myelin basic protein;myelin membrane encephalitogenic protein;proline-rich proteoglycan 2.
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reference: 1. Marty MC, Alliot F, Rutin J, Fritz R, Trisler D, Pessac B.The myelin basic protein gene is expressed
  in differentiated blood cell lineages and in hemopoietic progenitors. Proc Natl Acad Sci U S A. 2002
  Jun 25; 99(13):8856-61.
  2. Streicher R, Stoffel W.The organization of the human myelin basic protein gene. Comparison with
  the mouse gene. Biol Chem Hoppe Seyler. 1989 May; 370(5):503-10.
  3. McLaurin JA, Hafler DA, Antel JP.Reactivity of normal T-cell lines to MBP isolated from normal and
  multiple sclerosis white matter. J Neurol Sci. 1995 Feb; 128(2):205-11.
  4. Iester A, Vignola S, Callegarini L, Gimelli G, Alpigiani MG. [18q syndrome with deficiency of myelin
  basic protein (MBP)] Pediatr Med Chir. 1996 Mar-Apr; 18(2):201-5.
  5. Tranquill LR, Skinner E, Campagnoni C, Vergelli M, Hemmer B, Muraro P, Martin R, McFarland HF,
  Campagnoni AT, Voskuhl RR. Human T lymphocytes specific for the immunodominant 83-99
  epitope of myelin basic protein: recognition of golli MBP HOG 7. J Neurosci Res. 1996 Sep 15;
  45(6):820-8.
• Description of Target: The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Specificity: No cross reactivity with other proteins.
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
• Additional Information: Background: Myelin basic protein(MBP) is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the central nervous system and the peripheral nervous system, respectively. It is most abundant in hemopoietic system and contains seven exons distributed over 32-34 kb. MBP isolated from MS brain may differ in charge microheterogeneity which would affect antigenic determinants. MBP is mapped to chromosome 18q22-23. Failure in this gene expression would be correlated in the central white matter with extrapyramidal system degeneration signs. Moreover, it is a candidate autoantigen in the disease multiple sclerosis.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Mouse, Rat: Human: By Heat
  Immunofluorescence: 2 ug/ml: Mouse, Rat
• Immunogen: Polypeptide
• Dilution: Optimal dilutions should be determined by end user.
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Uniprot ID: P02686
• Protein Name: Myelin basic protein
• Publications:

  Cerebral Apolipoprotein-D Is Hypoglycosylated Compared to Peripheral Tissues and Is Variably Expressed in Mouse and Human Brain Regions. PLoS ONE. 11, e0148238 (2016). 26829325

• Purification: Affinity Purified
• Tested Species Reactivity: Human, Mouse, Rat
• Gene Symbol: MBP
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Application: Immunofluorescence|Immunohistochemistry|Western blot
• Image 1: Rat Brain
  
  IHC(P): Rat Brain Tissue
• Image 2: Mouse Brain
  
  WB: Mouse Brain Tissue Lysate