SARS-CoV-2 Nucleoprotein (N) (75G5a) Paired Antibody : Biotin (OAAQ00215)

• Product Number: OAAQ00215
• Name: SARS-CoV-2 Nucleoprotein (N) (75G5a) Paired Antibody : Biotin (OAAQ00215)
• Protein Size (# AA): 419 amino acids
• Molecular Weight: 46 kDa
• Clone: 75G5a
• Isotype: Rabbit IgGk
• Conjugation: Biotin
• NCBI Gene Id: 43740575
• Host: Rabbit
• Clonality: Monoclonal
• Concentration: 0.5 mg/mL
• Gene Full Name: nucleocapsid phosphoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Alias Symbols: NP, NC, N, ORF9
• Product Format: Liquid. 1X PBS, 0.02% NaN3
• Description of Target: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target. (Source NCBI). Paired with OAAQ00212 (84C4a) or OAAQ00214 (85C1), used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (N) in ELISA.
• Reconstitution and Storage: Store at 2-8C
• Additional Information: Antibody Pairing: Paired with OAAQ00212 (84C4a) or OAAQ00214 (85C1), used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (N).
• Uniprot ID: P0DTC9
• Protein Name: nucleocapsid phosphoprotein
• Protein Accession #: YP_009724397
• Purification: Protein A
• Nucleotide Accession #: NC_045512
• Gene Symbol: N
• Predicted Species Reactivity: SARS-CoV-2
• Application: ELISA
• Image 1: Western Blot
  
  Western blot analysis of SARS-CoV-2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL SARS-CoV-2 Nucleoprotein (N) (75G5a) Paired Antibody (OAAQ00211).
• Image 2: ELISA Titration
  
  Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (N) at 1 ug/mL. SARS-CoV-2 Nucleoprotein (N) (75G5a) Paired Antibody-Biotin (OAAQ00215) was serially diluted 1:2 starting at 1 ug/mL, and shows high-sensitivity binding to COVID-19 N antigen.
• Image 3: ELISA
  
  A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (N) specific rabbit monoclonal antibodies: 84C4a (OAAQ00212) as a capture antibody and 75G5a-biotin (OAAQ00215) as a detection antibody. COVID-19 N was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 84C4a and 75G5a-biotin detected COVID-19 N antigen at very high sensitivity as low as 49 pg/mL (4.9 pg).
• Image 4: ELISA
  
  A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (N) specific rabbit monoclonal antibodies: 85C1 (OAAQ00214) as a capture antibody and 75G5a-biotin (OAAQ00215) as a detection antibody. COVID-19 N was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 N antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).