SARS-CoV-2 Nucleoprotein (N) (85C1) Paired Antibody (OAAQ00214)

Data Sheet
Product Number OAAQ00214
Product Page
Name SARS-CoV-2 Nucleoprotein (N) (85C1) Paired Antibody (OAAQ00214)
Protein Size (# AA) 419 amino acids
Molecular Weight 46 kDa
Clone 85C1
Isotype Rabbit IgGk
Conjugation Unconjugated
NCBI Gene Id 43740575
Host Rabbit
Clonality Monoclonal
Concentration 0.5 mg/mL
Gene Full Name nucleocapsid phosphoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
Alias Symbols NP, NC, N, ORF9
Product Format 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% BSA
Description of Target Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target. (Source NCBI). Paired with OAAQ00211 (75G5a), used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (N) in ELISA.
Reconstitution and Storage Store at -20C
Datasheets/Manuals Printable datasheet for OAAQ00214
Additional Information Antibody Pairing: Paired with OAAQ00211 (75G5a), used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (N).
Uniprot ID P0DTC9
Protein Name nucleocapsid phosphoprotein
Protein Accession # YP_009724397
Purification Protein A
Nucleotide Accession # NC_045512
Gene Symbol N
Predicted Species Reactivity SARS-CoV-2
Application WB, ELISA
Image 1
Western Blot
Western blot analysis of 50 ng of SARS-CoV2 and SARS-CoV nucleocapsid protein probed with 1 ug/mL SARS-CoV-2 Nucleoprotein (N) (85C1) Paired Antibody (OAAQ00214).
Image 2
ELISA Titration
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (N) and SARS-CoV N at 1 ug/mL. Purified rabbit monoclonal antibody 85C1 (OAAQ00214) was serially diluted 1:2 starting at 1 ug/mL. SARS-CoV-2 Nucleoprotein (N) (85C1) Paired Antibody (OAAQ00214) showed very strong and specific binding to both COVID-19 N antigen and to SARS-CoV N antigen.
Image 3
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (N) specific rabbit monoclonal antibodies: 85C1 (OAAQ00214) as a capture antibody and 75G5a-biotin (OAAQ00215) as a detection antibody. COVID-19 N was serially diluted 1:2 starting at 25 ng/mL. Rabbit monoclonal antibodies 85C1 and 75G5a-biotin detected COVID-19 N antigen at very high sensitivity as low as 25 pg/mL (2.5 pg).

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