SARS-CoV-2 Nucleoprotein Antibody (OAAN06660)

• Product Number: OAAN06660
• Name: SARS-CoV-2 Nucleoprotein Antibody (OAAN06660)
• Isotype: IgG
• NCBI Gene Id: 1489678
• Host: Rabbit
• Clonality: Monoclonal
• Gene Full Name: nucleocapsid protein
• Alias Symbols: SARS-CoV-2 Nucleoprotein
• Product Format: Liquid PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Description of Target: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• Reconstitution and Storage: Store at 4°C. Avoid freeze / thaw cycles.
• Application Info: WB: 1:1000~5000
  ELISA: 1:1000~5000
  FC: 1:50~200
  IHC: 1:50~200
  IF: 1:50~200
  IP: 1:50~200
• Immunogen: Recombinant fusion protein of SARS-CoV-2 Nucleoprotein.
• Uniprot ID: P59595
• Protein Name: nucleocapsid protein
• Purification: Affinity purification
• Tested Species Reactivity: SARS-CoV-2
• Gene Symbol: N
• Application: WB, ELISA, FC, IHC, IF, IP
• Image 1: 2019-nCoV N Protein
  
  Western blot analysis of 2019-nCoV N Protein using 2019-nCoV N Protein Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.