| Product Number |
OAAN01583 |
| Product Page |
www.avivasysbio.com/srpk1-antibody-oaan01583.html |
| Name |
SRPK1 Rabbit pAb (OAAN01583) |
| Molecular Weight |
74kDa |
| Isotype |
IgG |
| Conjugation |
Unconjugated |
| NCBI Gene Id |
6732 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Gene Full Name |
SRSF protein kinase 1 |
| Alias Symbols |
Serine/arginine-rich protein-specific kinase 1;serine/arginine-rich splicing factor kinase 1;serine/threonine-protein kinase SRPK1;SFRS protein kinase 1;SFRSK1;SR-protein-specific kinase 1;SRSF protein kinase 1. |
| Product Format |
Liquid. PBS with 0.02% sodium azide, 50% glycerol, pH 7.3 |
| Description of Target |
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation. |
| Reconstitution and Storage |
Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Protein Sequence |
EEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLV |
| Datasheets/Manuals |
Printable datasheet for SRPK1 Rabbit pAb (OAAN01583) |
| Additional Information |
Cellular Location: Cytoplasm, Microsome, Microsome, Nucleus, Nucleus matrix
Positive Samples: 293T, HepG2, MCF7, HeLa, THP-1, Rat testis, Mouse spleen |
| Application Info |
WB: 1:500~2000
IHC: 1:50~200
IF: 1:10~100
|
| Immunogen |
SRPK1 |
| Intended Use |
For research purposes only. Not for therapeutic or diagnostic purposes in humans or animals. |
| Uniprot ID |
Q96SB4 |
| Protein Name |
SRSF protein kinase 1 |
| Protein Accession # |
NP_003128.3 |
| Purification |
Affinity purified against immunogen |
| Nucleotide Accession # |
NM_003137.4 |
| Gene Symbol |
SRPK1 |
| Predicted Species Reactivity |
Human, Mouse, Rat |
| Application |
Western blot |
| Image 1 | Multiple Cell Lines
 | Western blot analysis of extracts of various cell lines, using SRPK1 antibody. |
| | Image 2 | Human Kidney
 | Immunohistochemistry of paraffin-embedded human kidney cancer using SRPK1 antibody at dilution of 1:200 (400x lens). |
| | Image 3 | HeLa Cells,Human Kidney,Multiple Cell Lines
 | Immunofluorescence analysis of HeLa cell using SRPK1 antibody. Blue: DAPI for nuclear staining. |
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