IGF2BP1 Antibody (OAAJ06910)

• Product Number: OAAJ06910
• Name: IGF2BP1 Antibody (OAAJ06910)
• Molecular Weight: 63 kDa
• Isotype: IgG
• NCBI Gene Id: 10642
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 1 mg/mL
• Alias Symbols: IMP1, ZBP1, CRDBP, IMP-1, CRD-BP, VICKZ1
• Product Format: Liquid PBS (pH 7.4), 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Description of Target: Insulin-like growth factor-II mRNA-binding proteins (IMPs) belong to a family of zipcode-binding proteins. Three members of this family, IMP1, IMP2, and IMP3, have been identified. They contain two RNA recognition motifs, four K homology domains, and were found to function in mRNA localization, turnover, and translation control. Research studies have implicated these proteins in a variety of physiological and pathological processes, such as growth and development, testicular neoplasia, and melanocytic neoplasia.
• Reconstitution and Storage: Store at -20°C. Stable for 12 months from date of receipt.
• Specificity: IGF2BP1 Antibody detects endogenous levels of total IGF2BP1.
• Additional Information: Subcellular Location: Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments. Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines. Exhibited rapid, bidirectional movements in dendrites and spines. Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
  Tissue Specificity: Mainly expressed in the embryo, including in fetal liver, fetal lung, fetal kidney, fetal thymus (at protein level). Also expressed follicles of ovary, as well as in gonocytes of testis, spermatogonia, semen, oocytes and placenta (at protein level). Expressed in various cancers, including testis and lung cancers (at protein level), as well as kidney, prostate and trachea cancers.
• Application Info: WB: 1:500~2000
  IHC: 1:50~200
• Immunogen: A synthetic peptideof human IGF2BP1, corresponding to a region within N terminal amino acids.
• Uniprot ID: Q9NZI8
• Protein Name: Insulin-like growth factor 2 mRNA-binding protein 1
• Protein Accession #: NP_001153895
• Purification: Peptide affinity chromatography
• Nucleotide Accession #: NM_001160423
• Tested Species Reactivity: Human, Mouse, Rat
• Gene Symbol: IGF2BP1
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
• Image 1: various cell lines
  
  Western blot analysis of extracts of variouscell lines, using IGF2BP1 antibody.