| Product Number |
OAAI00757 |
| Product Page |
www.avivasysbio.com/anti-zbtb4-antibody-nt-polyclonal-antibody-oaai00757.html |
| Name |
Anti-ZBTB4 Antibody (NT) Polyclonal Antibody (OAAI00757) |
| Molecular Weight |
105 |
| Isotype |
Rabbit IgG |
| NCBI Gene Id |
57659 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
100ug/200ul |
| Gene Full Name |
zinc finger and BTB domain containing 4 |
| Alias Symbols |
KAISO-L1;KAISO-like zinc finger protein 1;zinc finger and BTB domain-containing protein 4;ZNF903. |
| Product Format |
Liquid. This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Reference |
Axel Weber, et al. ZBTB4 represses transcription of P21CIP1 and controls the cellular response to p53 activation. The EMBO Journal, 27, 1563 - 1574 (2008). |
| Description of Target |
Zink finger and BTB domain coating 4 (ZBTB4) is a transcriptional repressor protein, which is associated with the Sin3/histone deacetylase co-repressor and represses expression of P21CIP1 as part of a heterodimeric complex with Miz1.
In vivo, expression of ZBTB4 is downregulated in advanced stages of multiple Human tumours. In cell culture, depletion of ZBTB4 promotes cell cycle arrest in response to activation of p53 and suppresses apoptosis throμgh regulation of P21CIP1, thereby promoting long-term cell survival. ZBTB4 is a critical determinant of the cellular response to p53 activation and reinforce the notion that p21Cip1 can provide an essential survival signal in cells with activated p53.
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| Reconstitution and Storage |
The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles. |
| Datasheets/Manuals |
Printable datasheet for Anti-ZBTB4 Antibody (NT) Polyclonal Antibody (OAAI00757) |
| Specificity |
ZBTB4 |
| Additional Information |
Background: Zink finger and BTB domain coating 4 (ZBTB4) is a transcriptional repressor protein, which is associated with the Sin3/histone deacetylase co-repressor and represses expression of P21CIP1 as part of a heterodimeric complex with Miz1. In vivo, expression of ZBTB4 is downregulated in advanced stages of multiple human tumours. In cell culture, depletion of ZBTB4 promotes cell cycle arrest in response to activation of p53 and suppresses apoptosis through regulation of P21CIP1, thereby promoting long-term cell survival. ZBTB4 is a critical determinant of the cellular response to p53 activation and reinforce the notion that p21Cip1 can provide an essential survival signal in cells with activated p53. |
| Additional Information |
Positive Control: Brain tissue |
| Application Info |
WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested |
| Immunogen |
A synthetic peptide corresponding to the N-terminus of human ZBTB4 protein. This sequence is identical to human, mouse, rat, bovine, canis etc. |
| Additional Information |
Cellular Location: Cytoplasmic/Nuclear |
| Formulation |
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer |
| Uniprot ID |
Q9P1Z0 |
| Purification |
Affinity Purified |
| Gene Symbol |
ZBTB4 |
| Predicted Species Reactivity |
Bovine|Human|Mouse|Rat |
| Application |
Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot |
| Image 1 | Mouse Brain
 | Western Blot: The whole mouse brain tissue lysates were immunoblotted by Rabbit anti-ZBTB4 (Cat#OAAI00757) at 1:500 (Lane 1). The immunoreactive band is blocked by pre-incubation of immunizing peptide (lane 2) |
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