Anti- Beta -Catenin Antibody (Phospho-S37) (OAAI00597)

• Product Number: OAAI00597
• Name: Anti- Beta -Catenin Antibody (Phospho-S37) (OAAI00597)
• Molecular Weight: 100
• Isotype: Rabbit IgG
• NCBI Gene Id: 1499
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 100ug/200ul
• Gene Full Name: catenin beta 1
• Alias Symbols: armadillo;catenin (cadherin-associated protein), beta 1, 88kDa;catenin beta-1;CTNNB;EVR7;MRD19;NEDSDV.
• Product Format: Liquid. This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Reference: Park, C.S. et al. (2004) J. Biol. Chem. 279:19592.
• Description of Target: Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the beta-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, beta-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3beta and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates beta-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of beta-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3beta activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3beta, allowing unphosphorylated beta-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
  
• Reconstitution and Storage: The antibodies are stable for 24 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C-8C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
• Specificity: CTNNB1
• Additional Information: Background: Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the ?-catenin family. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It is believed to contribute to tight cell-to-cell adhesion. It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, ?-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3? and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates ?-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of ?-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3? activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3?, allowing unphosphorylated ?-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis.
• Additional Information: Positive Control: Human lung adenocarcinoma
• Application Info: WB: 0.1-1 ug/ml | ELISA: 0.01-0.1 ug/ml | IP: 2-5 ug/ml | IHC: 2-10 ug/ml | FC: Not tested
• Immunogen: A synthetic peptide corresponding to the epitope SGIHS with a single phosphorylation site Ser37 of human -catenin.
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
• Formulation: This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
• Uniprot ID: P35222
• Purification: Affinity Purified
• Target Post-Translational Modification: Phosphorylated
• Gene Symbol: CTNNB1
• Predicted Species Reactivity: Human|Mouse|Rat
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunohistochemistry|Immunoprecipitation|Western blot
• Image 1: Hela
  
  Western Blot: The cell lysate derived from HELA was immunoprobed at a dilution of 1:500 by the following antibodies: 1: Rabbit anti-beta-Catenin (pS33/S37) (Cat#OAAI00538) 2: Rabbit anti-beta-Catenin (pS33) (Cat#OAAI00537) 3: Rabbit anti-beta-Catenin (pS37) (Cat#OAAI00597) 4: Rabbit anti-beta-Catenin (Non-phospho) (Cat#OAAI00596) 5: Negative control.
• Image 2: Phospho-peptide, non-phospho-peptide
  
  Dot Blot: 1 ?g peptide was blot onto NC membrane A: Beta-Catenin (pS33/S37) B: Beta-Catenin (pS33) C: Beta-Catenin (pS37) D: Beta-Catenin (Nonphospho); E: Non-related Phosphopeptide) were blotted at a 1:1000 dilution by: 1: Rabbit anti-beta-Catenin (pS33/S37) (Cat#OAAI00538) 2: Rabbit anti-beta-Catenin (pS33) (Cat#OAAI00537) 3: Rabbit anti-beta-Catenin (pS37) (Cat#OAAI00597) 4: Rabbit anti-beta-Catenin (Non- phospho) (Cat#OAAI00596)